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Deep mutational scanning for therapeutic antibody engineering
Trends in Pharmacological Sciences, 2022The biophysical and functional properties of monoclonal antibody (mAb) drug candidates are often improved by protein engineering methods to increase the probability of clinical efficacy. One emerging method is deep mutational scanning (DMS) which combines the power of exhaustive protein mutagenesis and functional screening with deep sequencing and ...
Kyrin R, Hanning +4 more
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Intragenic compensation through the lens of deep mutational scanning
Biophysical Reviews, 2022A significant fraction of mutations in proteins are deleterious and result in adverse consequences for protein function, stability, or interaction with other molecules. Intragenic compensation is a specific case of positive epistasis when a neutral missense mutation cancels effect of a deleterious mutation in the same protein.
Nadezhda Azbukina +2 more
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Flavivirus deep mutational scanning
2023This is Matthew Evan’s presentation at the monthly [virtual] Antiviral Drug Discovery (AViDD) Open Science Forum on 2023 January 18. You can find a recording of their talk on the AViDD Open Science Forum Youtube page. Matthew Evans is a part of the ASAP Discovery Consortium.
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Biological fitness landscapes by deep mutational scanning
2020Knowledge of the distribution of fitness effects (DFE) of mutations is critical to the understanding of protein evolution. Here, we describe methods for large-scale, systematic measurements of the DFE using growth competition and deep mutational scanning.
Jacob D, Mehlhoff, Marc, Ostermeier
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Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display
2022Protein engineering using display platforms such as yeast display and phage display has allowed discovery of proteins with therapeutic and industrial applications. Antibodies and T cell receptors developed for therapeutic applications are often engineered by constructing libraries of mutations in loops of five to ten residues called complementarity ...
Preeti, Sharma +2 more
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Application of Deep Mutational Scanning in Hepatitis C Virus
2018Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the ...
Nicholas C, Wu, Hangfei, Qi
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Deep Eutectic Solvents: A Review of Fundamentals and Applications
Chemical Reviews, 2021Brian Chen, Yong Zhang, Jeffrey M Klein
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Soft wearable devices for deep-tissue sensing
Nature Reviews Materials, 2022Muyang Lin, Hongjie Hu, Sai Zhou
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Deep neural networks for the evaluation and design of photonic devices
Nature Reviews Materials, 2020Jiaqi Jiang +2 more
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