Results 21 to 30 of about 19,763 (281)
Mutational fitness landscape of human influenza H3N2 neuraminidase
Cell Reports, 2023 Summary: Influenza neuraminidase (NA) has received increasing attention as an effective vaccine target. However, its mutational tolerance is not well characterized.
Ruipeng Lei +9 more
doaj +1 more source
Structural and functional determinants inferred from deep mutational scans [PDF]
Protein Science, 2022 Abstract Mutations that affect protein binding to a cognate partner primarily occur either at buried residues or at exposed residues directly involved in partner binding. Distinguishing between these two categories based solely on mutational phenotypes is challenging. The bacterial toxin CcdB kills cells by binding to DNA Gyrase. Cell
Priyanka Bajaj +2 more
openaire +2 more sources
Deep Mutational Scan of an SCN5A Voltage Sensor [PDF]
Circulation: Genomic and Precision Medicine, 2020 Background: Variants in ion channel genes have classically been studied in low throughput by patch clamping. Deep mutational scanning is a complementary approach that can simultaneously assess function of thousands of variants. Methods: We have developed and validated
Glazer, Andrew +8 more
openaire +5 more sources
Exploring the origins of nucleation
eLife, 2021 An approach called deep mutational scanning is improving our understanding of amyloid beta aggregation.
Katarzyna Marta Zoltowska +1 more
doaj +1 more source
Rational Protein Engineering Guided by Deep Mutational Scanning [PDF]
International Journal of Molecular Sciences, 2015 Sequence–function relationship in a protein is commonly determined by the three-dimensional protein structure followed by various biochemical experiments. However, with the explosive increase in the number of genome sequences, facilitated by recent advances in sequencing technology, the gap between protein sequences available and three-dimensional ...
Shin, HyeonSeok, Cho, Byung-Kwan
openaire +2 more sources
Inferring protein 3D structure from deep mutation scans [PDF]
Nature Genetics, 2019 We describe an experimental method of three-dimensional (3D) structure determination that exploits the increasing ease of high-throughput mutational scans. Inspired by the success of using natural, evolutionary sequence covariation to compute protein and RNA folds, we explored whether 'laboratory', synthetic sequence variation might also yield 3D ...
Nathan J. Rollins +6 more
openaire +3 more sources
Fitness landscape of a dynamic RNA structure.
PLoS Genetics, 2021 RNA structures are dynamic. As a consequence, mutational effects can be hard to rationalize with reference to a single static native structure. We reasoned that deep mutational scanning experiments, which couple molecular function to fitness, should ...
Valerie W C Soo +3 more
doaj +1 more source
Visualizing Deep Mutational Scan Data [PDF]
, 2018 AbstractSite-directed and random mutagenesis are biochemical tools to obtain insights into the structure and function of proteins. Recent advances such as deep mutational scan have allowed a complete scan of all the amino acid positions in a protein with each of the 19 possible alternatives.
Sruthi, C. K. +2 more
openaire +1 more source
eLife, 2022 A fundamental question in protein science is where allosteric hotspots – residues critical for allosteric signaling – are located, and what properties differentiate them.
Megan Leander +3 more
doaj +1 more source
Highly Mutable Linker Regions Regulate HIV-1 Rev Function and Stability. [PDF]
, 2019 HIV-1 Rev is an essential viral regulatory protein that facilitates the nuclear export of intron-containing viral mRNAs. It is organized into structured, functionally well-characterized motifs joined by less understood linker regions.
Fernandes, Jason D +4 more
core +3 more sources