Results 31 to 40 of about 19,763 (281)
Unsupervised Inference of Protein Fitness Landscape from Deep Mutational Scan [PDF]
Molecular Biology and Evolution, 2020 Abstract The recent technological advances underlying the screening of large combinatorial libraries in high-throughput mutational scans deepen our understanding of adaptive protein evolution and boost its applications in protein design.
Fernandez-De-Cossio-Diaz, Jorge +2 more
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Exploring the functional residues in a flavin-binding fluorescent protein using deep mutational scanning. [PDF]
PLoS ONE, 2014 Flavin mononucleotide (FMN)-based fluorescent proteins are versatile reporters that can monitor various cellular processes in both aerobic and anaerobic conditions.
HyeonSeok Shin +6 more
doaj +1 more source
Frontiers in Molecular Biosciences, 2021 Mutational scanning can be used to probe effects of large numbers of point mutations on protein function. Positions affected by mutation are primarily at either buried or at exposed residues directly involved in function, hereafter designated as active ...
Munmun Bhasin +2 more
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Dissecting enzyme function with microfluidic-based deep mutational scanning [PDF]
Proceedings of the National Academy of Sciences, 2015 Significance As powerful biological catalysts, enzymes can solve challenging problems that range from the industrial production of chemicals to the treatment of human disease. The ability to design new enzymes with tailor-made chemical functions would have a far-reaching impact.
Romero, Philip A +2 more
openaire +4 more sources
Ion-ing out the genetic variants of Kir2.1
eLife, 2022 Deep mutational scanning provides new insights into how mutations alter the expression and activity of the potassium ion channel Kir2.1, which is associated with many diseases.
Braden S Fallon, Justin G English
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Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data [PDF]
, 2015 There is a high prevalence of coronary artery disease (CAD) in patients with left bundle branch block (LBBB); however there are many other causes for this electrocardiographic abnormality.
Haydon, Daniel T. +6 more
core +2 more sources
Nonproteinogenic deep mutational scanning of linear and cyclic peptides [PDF]
Proceedings of the National Academy of Sciences, 2018 Significance The 20 proteinogenic amino acids have physicochemical properties that allow peptides and proteins to fold and bind. However, there are numerous unnatural, nonproteinogenic amino acids that may be equally good, or even better, at folding and binding. Exploration of these alternative peptide building blocks has been limited by slow,
Rogers, Joseph M. +2 more
openaire +2 more sources
Globally defining the effects of mutations in a picornavirus capsid
eLife, 2021 The capsids of non-enveloped viruses are highly multimeric and multifunctional protein assemblies that play key roles in viral biology and pathogenesis. Despite their importance, a comprehensive understanding of how mutations affect viral fitness across ...
Florian Mattenberger +4 more
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Hot-spot analysis for drug discovery targeting protein-protein interactions [PDF]
, 2018 Introduction: Protein-protein interactions are important for biological processes and pathological situations, and are attractive targets for drug discovery. However, rational drug design targeting protein-protein interactions is still highly challenging.
Fernández-Recio, Juan, Rosell, Mireia
core +2 more sources
Globally Defining the Effects of Amino Acid Mutations across a Picornavirus Capsid
Proceedings, 2020 RNA viruses are characterized by their extreme mutation rates, which play key roles in their biology and give them the ability to rapidly adapt to new environments.
Florian Mattenberger, Ron Geller
doaj +1 more source