Deep mutational scanning of RNA polymerase
, 2023 RNA polymerase deep mutational scanning data accompanying the manuscript titled: Deep mutational scanning reveals the molecular determinants of RNA polymerase-mediated adaptation and ...
Choudhury, Alaksh, Tenaillon, Olivier
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Activation-induced deoxycytidine deaminase (AID) co-transcriptional scanning at single-molecule resolution [PDF]
, 2015 Activation-induced deoxycytidine deaminase (AID) generates antibody diversity in B cells by initiating somatic hypermutation (SHM) and class-switch recombination (CSR) during transcription of immunoglobulin variable (IgV) and switch region (IgS) DNA ...
Bertram, JG +7 more
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Exploring amino acid functions in a deep mutational landscape
Molecular Systems Biology, 2021 Amino acids fulfil a diverse range of roles in proteins, each utilising its chemical properties in different ways in different contexts to create required functions.
Alistair S Dunham, Pedro Beltrao
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TITER: predicting translation initiation sites by deep learning. [PDF]
, 2017 MotivationTranslation initiation is a key step in the regulation of gene expression. In addition to the annotated translation initiation sites (TISs), the translation process may also start at multiple alternative TISs (including both AUG and non-AUG ...
Hu, Hailin +4 more
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A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]
, 2020 Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A. +15 more
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Interpreting protein variant effects with computational predictors and deep mutational scanning
Disease Models & Mechanisms, 2022 Computational predictors of genetic variant effect have advanced rapidly in recent years. These programs provide clinical and research laboratories with a rapid and scalable method to assess the likely impacts of novel variants.
Benjamin J. Livesey, Joseph A. Marsh
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Deep mutational scanning: a new style of protein science [PDF]
Nature Methods, 2014 Mutagenesis provides insight into proteins, but only recently have assays that couple genotype to phenotype been used to assess the activities of as many as 1 million mutant versions of a protein in a single experiment. This approach-'deep mutational scanning'-yields large-scale data sets that can reveal intrinsic protein properties, protein behavior ...
Douglas M, Fowler, Stanley, Fields
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Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]
, 2020 Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL +3 more
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Rapid, ultra low coverage copy number profiling of cell-free DNA as a precision oncology screening strategy. [PDF]
, 2017 Current cell-free DNA (cfDNA) next generation sequencing (NGS) precision oncology workflows are typically limited to targeted and/or disease-specific applications.
Alva, Ajjai S +31 more
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Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape
Scientific Reports, 2021 The serine protease inhibitor (SERPIN) plasminogen activator inhibitor-1 (PAI-1) is a key regulator of the fibrinolytic system, inhibiting the serine proteases tissue- and urokinase-type plasminogen activator (tPA and uPA, respectively).
Zachary M. Huttinger +7 more
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