Transfer RNA-derived small RNAs in the cancer transcriptome [PDF]
, 2016 The cellular lifetime includes stages such as differentiation, proliferation, division, senescence and apoptosis.These stages are driven by a strictly ordered process of transcription dynamics.
A Dueck +38 more
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Biology Open, 2018 Although the primary protein sequence of ubiquitin (Ub) is extremely stable over evolutionary time, it is highly tolerant to mutation during selection experiments performed in the laboratory.
David Mavor +64 more
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Accurate Measurement of the Effects of All Amino-Acid Mutations on Influenza Hemagglutinin
Viruses, 2016 Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths available to the virus.
Michael B. Doud, Jesse D. Bloom
doaj +1 more source
Mapping mutations to the SARS-CoV-2 RBD that escape binding by different classes of antibodies
Nature Communications, 2021 Emerging SARS-CoV-2 mutants may escape neutralization by antibodies. Here, the authors use deep mutational scanning to identify mutations in the RBD that escape human monoclonal antibodies or convalescent plasmas.
Allison J. Greaney +18 more
doaj +1 more source
Enzyme engineering in biomimetic compartments. [PDF]
, 2015 The success of a directed evolution approach to creating custom-made enzymes relies in no small part on screening as many clones as possible. The miniaturisation of assays into pico to femtoliter compartments (emulsion droplets, vesicles or gel-shell ...
Colin, Pierre-Yves +2 more
core +2 more sources
Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. [PDF]
, 2016 Rocaglamide A (RocA) typifies a class of protein synthesis inhibitors that selectively kill aneuploid tumour cells and repress translation of specific messenger RNAs.
Floor, Stephen N +2 more
core +1 more source
dms-view: Interactive visualization tool for deep mutational scanning data [PDF]
Journal of Open Source Software, 2020 Summary and PurposeThe high-throughput technique of deep mutational scanning (DMS) has recently made it possible to experimentally measure the effects of all amino-acid mutations to a protein (Fowler and Fields 2014). Over the past five years, this technique has been used to study dozens of different proteins (Esposito et al. 2019) and answer a variety
Sarah Hilton +6 more
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A mechanistic explanation linking adaptive mutation, niche change, and fitness advantage for the Wrinkly Spreader [PDF]
, 2014 Experimental evolution studies have investigated adaptive radiation in static liquid microcosms using the environmental bacterium Pseudomonas fluorescens SBW25.
Spiers, Andrew J.
core +5 more sources
Using deep mutational scanning to benchmark variant effect predictors and identify disease mutations [PDF]
Molecular Systems Biology, 2019 AbstractTo deal with the huge number of novel protein-coding variants identified by genome and exome sequencing studies, many computational variant effect predictors (VEPs) have been developed. Such predictors are often trained and evaluated using different variant datasets, making a direct comparison between VEPs difficult.
Benjamin J. Livesey, Joseph A. Marsh
openaire +3 more sources
Allo-network drugs: Extension of the allosteric drug concept to protein-protein interaction and signaling networks [PDF]
, 2013 Allosteric drugs are usually more specific and have fewer side effects than orthosteric drugs targeting the same protein. Here, we overview the current knowledge on allosteric signal transmission from the network point of view, and show that most ...
Csermely, Péter +2 more
core +2 more sources