Results 61 to 70 of about 19,763 (281)

Deep mutational scanning: assessing protein function on a massive scale [PDF]

Trends in Biotechnology, 2011
Analysis of protein mutants is an effective means to understand their function. Protein display is an approach that allows large numbers of mutants of a protein to be selected based on their activity, but only a handful with maximal activity have been traditionally identified for subsequent functional analysis.
Carlos L, Araya, Douglas M, Fowler
openaire   +2 more sources

SerpinB2 regulates stromal remodelling and local invasion in pancreatic cancer [PDF]

, 2017
Pancreatic cancer has a devastating prognosis, with an overall 5-year survival rate of ~8%, restricted treatment options and characteristic molecular heterogeneity.
A H Allam, A Neesse, A Neesse, A V Biankin, A Xue, AB Seymour, AD Rhim, AK Witkiewicz, AM LeBeau, AR Shekouh, AV Biankin, B Hinz, BC Ozdemir, BJ Cochran, BJ Cochran, C Gaggioli, C Vennin, D Croucher, D Goldstein, D Herrmann, D Herrmann, D Mahadevan, D N Saunders, D Telgenhoff, DD Von Hoff, DR Croucher, DR Croucher, E Cerami, F Al-Ejeh, G Finak, H Shiomi, HW Smith, I Garrido-Laguna, J Condeelis, J Gao, J Gore, J Lee, J Li, J P Morton, J R W Conway, JA Lee, JJ Lee, JP Morton, K Kessenbrock, K L Vine, L Harris, M C Lucas, M J Cowley, M Nobis, M Pajic, M Pinese, M Ranson, MJ Duffy, ML Nyström, N L E Harris, N Waddell, P Bailey, P Carmeliet, P Timpson, P Timpson, R Cicchi, R F Shearer, R Kalluri, R Smith, RF Shearer, RG Rowe, RL Siegel, S Nurmenniemi, S Ulisse, SC Barry, SR Hingorani, SR Hingorani, TK Stutchbury, WA Schroder, Y Takeuchi, ZS Boyd, Ö Babur   +76 more
core   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Measuring the sequence-affinity landscape of antibodies with massively parallel titration curves [PDF]

, 2016
Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody's amino acid sequence and its antigen binding affinity.
Adams, R. M., Kinney, J. B., Mora, T., Walczak, A. M.   +3 more
core   +2 more sources

Uncovering the basis of protein-protein interaction specificity with a combinatorially complete library

eLife, 2020
Protein-protein interaction specificity is often encoded at the primary sequence level. However, the contributions of individual residues to specificity are usually poorly understood and often obscured by mutational robustness, sequence degeneracy, and ...
Thuy-Lan V Lite, Robert A Grant, Isabel Nocedal, Megan L Littlehale, Monica S Guo, Michael T Laub   +5 more
doaj   +1 more source

Deep mutational scanning highlights a role for cytosolic regions in Hrd1 function

Cell Reports, 2023
Summary: Misfolded endoplasmic reticulum (ER) proteins are degraded through a process called ER-associated degradation (ERAD). Soluble, lumenal ERAD targets are recognized, retrotranslocated across the ER membrane, ubiquitinated, extracted from the ...
Brian G. Peterson, Jiwon Hwang, Jennifer E. Russ, Jeremy W. Schroeder, P. Lydia Freddolino, Ryan D. Baldridge   +5 more
doaj   +1 more source

MPRAnalyze: statistical framework for massively parallel reporter assays. [PDF]

, 2019
Massively parallel reporter assays (MPRAs) can measure the regulatory function of thousands of DNA sequences in a single experiment. Despite growing popularity, MPRA studies are limited by a lack of a unified framework for analyzing the resulting data ...
Ahituv, Nadav, Ashuach, Tal, Fischer, David S, Kreimer, Anat, Theis, Fabian J, Yosef, Nir   +5 more
core  

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Deep Mutational Scanning to Predict Escape from Bebtelovimab in SARS-CoV-2 Omicron Subvariants

Vaccines, 2023
The major concern with COVID-19 therapeutic monoclonal antibodies is the loss of efficacy against continuously emerging variants of SARS-CoV-2. To predict antibody efficacy against future Omicron subvariants, we conducted deep mutational scanning (DMS ...
Mellissa C. Alcantara, Yusuke Higuchi, Yuhei Kirita, Satoaki Matoba, Atsushi Hoshino   +4 more
doaj   +1 more source