Results 71 to 80 of about 19,763 (281)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. [PDF]

, 2019
The interplay between a highly polymorphic set of MHC-I alleles and molecular chaperones shapes the repertoire of peptide antigens displayed on the cell surface for T cell surveillance.
Choi, Hannah, Devlin, Christine A, Flores-Solis, David, McShan, Andrew C, Moschidi, Danai, Overall, Sarah A, Park, Jihye, Procko, Erik, Sgourakis, Nikolaos G, Toor, Jugmohit S, Tripathi, Sarvind   +10 more
core  

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Enriching Peptide Libraries for Binding Affinity and Specificity Through Computationally Directed Library Design [PDF]

, 2018
Peptide reagents with high affinity or specificity for their target protein interaction partner are of utility for many important applications. Optimization of peptide binding by screening large libraries is a proven and powerful approach.
A Stein, AM Levin, AS Parker, BA Liu, BD Allen, CL Araya, CL Kingsford, D Verma, DM Fowler, DM Fowler, DM Fowler, DW Sammond, E Procko, E-M Strauch, F Zheng, G Fernandez-Ballester, G Guntas, GW Foight, HJ Olivos, IE Sánchez, J DeBartolo, J DeBartolo, KE Roberts, LM Starita, M Boersma, M Goldsmith, MA Mena, MMY Chen, N London, N London, N London, RJ Hayes, RJ Pantazes, S Dutta, S Sirin, SJ Fleishman, T Hou, TM Jacobs, TP Treynor, TS Chen, TS Chen, X Fu   +41 more
core   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Deep mutational scanning of influenza A virus neuraminidase facilitates the identification of drug resistance mutations in vivo

mSystems, 2023
Neuraminidase (NA) is a pivotal surface enzyme and a key therapeutic target in combating the influenza A virus. Its evolution can lead to potential zoonotic transmission, seasonal epidemics, and the emergence of drug-resistant mutants.
Sihan Wang, Tian-hao Zhang, Menglong Hu, Kejun Tang, Li Sheng, Mengying Hong, Dongdong Chen, Liubo Chen, Yuan Shi, Jun Feng, Jing Qian, Lifeng Sun, Kefeng Ding, Ren Sun, Yushen Du   +14 more
doaj   +1 more source

Saturation mutagenesis reveals manifold determinants of exon definition. [PDF]

, 2017
To illuminate the extent and roles of exonic sequences in the splicing of human RNA transcripts, we conducted saturation mutagenesis of a 51-nt internal exon in a three-exon minigene.
Anquetil, Vincent, Arias, Mauricio A., Chasin, Lawrence A., Ju, Jingyue, Kalachikov, Sergey, Ke, Shengdong, Maity, Alisha, Russo, James J, Yang, Anthony, Zamalloa, Jorge Rojas   +9 more
core   +2 more sources

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

Phenotypic pleiotropy of missense variants in human B cell confinement receptor P2RY8

Cell Genomics
Summary: Missense variants can have pleiotropic effects on protein function, and predicting these effects can be difficult. We performed near-saturation deep mutational scanning of P2RY8, a G protein-coupled receptor that promotes germinal center B cell ...
Taylor N. LaFlam, Christian B. Billesbølle, Tuan Dinh, Finn D. Wolfreys, Erick Lu, Tomas Matteson, Jinping An, Ying Xu, Arushi Singhal, Nadav Brandes, Vasilis Ntranos, Aashish Manglik, Jason G. Cyster, Chun Jimmie Ye   +13 more
doaj   +1 more source

Identification of HIV-1 Envelope Mutations that Enhance Entry Using Macaque CD4 and CCR5

Viruses, 2020
Although Rhesus macaques are an important animal model for HIV-1 vaccine development research, most transmitted HIV-1 strains replicate poorly in macaque cells. A major genetic determinant of this species-specific restriction is a non-synonymous mutation
Jeremy I. Roop, Noah A. Cassidy, Adam S. Dingens, Jesse D. Bloom, Julie Overbaugh   +4 more
doaj   +1 more source