Results 21 to 30 of about 240,068 (314)
eLife, 2018 Hematopoietic stem cells require MLL1, which is one of six Set1/Trithorax-type histone 3 lysine 4 (H3K4) methyltransferases in mammals and clinically the most important leukemia gene. Here, we add to emerging evidence that all six H3K4 methyltransferases
Kerstin Schmidt +10 more
doaj +1 more source
Quantitative phenotyping via deep barcode sequencing [PDF]
Genome Research, 2009 Next-generation DNA sequencing technologies have revolutionized diverse genomics applications, including de novo genome sequencing, SNP detection, chromatin immunoprecipitation, and transcriptome analysis. Here we apply deep sequencing to genome-scale fitness profiling to evaluate yeast strain collections in parallel. This method,
Andrew M, Smith +8 more
openaire +2 more sources
Library construction for next-generation sequencing: Overviews and challenges
BioTechniques, 2014 High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing ...
Steven R. Head +6 more
doaj +1 more source
Shorter TCR β-Chains Are Highly Enriched During Thymic Selection and Antigen-Driven Selection
Frontiers in Immunology, 2019 The adaptive immune system uses several strategies to generate a repertoire of T cell receptors (TCR) with sufficient diversity to recognize the universe of potential pathogens.
Xianliang Hou +8 more
doaj +1 more source
AlphaSeq: Sequence Discovery With Deep Reinforcement Learning [PDF]
IEEE Transactions on Neural Networks and Learning Systems, 2020 48 pages, 13 ...
Yulin Shao +2 more
openaire +3 more sources
Frontiers in Microbiology, 2017 Characterization of newly isolated mycoviruses may contribute to understanding of the evolution and diversity of viruses. Here, a deep sequencing approach was used to analyze the double-stranded RNA (dsRNA) mycoviruses isolated from field-collected P ...
Li Zheng +8 more
doaj +1 more source
Linear amplification for deep sequencing
Nature Protocols, 2011 Linear amplification for deep sequencing (LADS) is an amplification method that produces representative libraries for Illumina next-generation sequencing within 2 d. The method relies on attaching two different sequencing adapters to blunt-end repaired and A-tailed DNA fragments, wherein one of the adapters is extended with the sequence for the T7 RNA ...
Hoeijmakers, W.A.M. +3 more
openaire +4 more sources
Understanding sequence conservation with deep learning [PDF]
Proceedings of the 8th ACM International Conference on Bioinformatics, Computational Biology,and Health Informatics, 2017 AbstractMotivationComparing the human genome to the genomes of closely related mammalian species has been a powerful tool for discovering functional elements in the human genome. Millions of conserved elements have been discovered. However, understanding the functional roles of these elements still remain a challenge, especially in noncoding regions ...
Li, Yi, Quang, Daniel, Xie, Xiaohui
openaire +1 more source
Deep Sequence Analysis of AgoshRNA Processing Reveals 3’ A Addition and Trimming
Molecular Therapy: Nucleic Acids, 2015 The RNA interference (RNAi) pathway, in which microprocessor and Dicer collaborate to process microRNAs (miRNA), was recently expanded by the description of alternative processing routes. In one of these noncanonical pathways, Dicer action is replaced by
Alex Harwig +4 more
doaj +1 more source
Genetics and Molecular Biology, 2012 The legume Glycine max (soybean) plays an important economic role in the international commodities market, with a world production of almost 260 million tons for the 2009/2010 harvest.
Ramon Oliveira Vidal +4 more
doaj +1 more source