Results 31 to 40 of about 240,068 (314)
VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)
Frontiers in Bioengineering and Biotechnology, 2023 The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda +13 more
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From deep sequencing to actual clones [PDF]
Protein Engineering Design and Selection, 2014 The application of deep sequencing to in vitro display technologies has been invaluable for the straightforward analysis of enriched clones. After sequencing in vitro selected populations, clones are binned into identical or similar groups and ordered by abundance, allowing identification of those that are most enriched.
Sara, D'Angelo +5 more
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MicroRNA Expression Profiling in Newcastle Disease Virus-Infected DF-1 Cells by Deep Sequencing
Frontiers in Microbiology, 2019 Newcastle disease virus (NDV), causative agent of Newcastle disease (ND), is one of the most devastating pathogens for poultry industry worldwide.
Yu Chen +19 more
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Viruses, 2019 Peste des petits ruminants (PPR) is one of the highly contagious transboundary viral diseases of small ruminants. Host microRNA (miRNA) expression patterns may change in response to virus infection, and it mainly works as a post-transcriptional moderator
Yang Yang +6 more
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NGS-based deep bisulfite sequencing
MethodsX, 2016 We have developed an NGS-based deep bisulfite sequencing protocol for the DNA methylation analysis of genomes. This approach allows the rapid and efficient construction of NGS-ready libraries with a large number of PCR products that have been individually amplified from bisulfite-converted DNA.
Lee, Suman, Kim, Joomyeong
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Full Genome of Influenza A (H7N9) Virus Derived by Direct Sequencing without Culture
Emerging Infectious Diseases, 2013 An epidemic caused by influenza A (H7N9) virus was recently reported in China. Deep sequencing revealed the full genome of the virus obtained directly from a patient’s sputum without virus culture.
Xianwen Ren +12 more
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Targeted deep sequencing in primary myelofibrosis [PDF]
Blood Advances, 2016 Key Points More than 80% of patients with PMF harbor DNA variants/mutations other than JAK2/CALR/MPL. Some of these variants/mutations adversely affect overall or leukemia-free survival independent of conventional risk stratification.
Ayalew Tefferi +7 more
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Frontiers in Genetics, 2022 Background: Androgen insensitivity syndrome (AIS) is an X-linked recessive hereditary disease caused due to a reduced or absent function of the androgen receptor (AR) protein encoded by the AR gene (OMIM-Gene# 313,700).
Xiaojing He +9 more
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Viroid quasispecies revealed by deep sequencing [PDF]
RNA Biology, 2017 Viroids are non-coding single-stranded circular RNA molecules that replicate autonomously in infected host plants causing mild to lethal symptoms. Their genomes contain about 250–400 nucleotides, depending on viroid species. Members of the family Pospiviroidae, like the Potato spindle tuber viroid (PSTVd), replicate via an asymmetric rolling-circle ...
Joseph R. J. Brass +3 more
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Analysis of the Sequence Preference of Saporin by Deep Sequencing
ACS Chemical Biology, 2022 Ribosome-inactivating proteins (RIPs) are RNA:adenosine glycosidases that inactivate eukaryotic ribosomes by depurinating the sarcin-ricin loop (SRL) in 28S rRNA. The GAGA sequence at the top of the SRL or at the top of a hairpin loop is assumed to be their target motif.
Samuel Hauf +2 more
openaire +2 more sources