Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
Chrono-Colonic Delivery in Engineering Time-Responsive Systems for Site-Specific Therapy
Background: Chrono-colonic drug delivery represents a novel pharmaceutical engineering strategy that combines the principles of circadian rhythm-based chronotherapy with colon-targeted the drug delivery.
Mukherjee, Deepannita Roy +6 more
core +1 more source
The anesthesia-to-incision interval accounts for most of the observed difference in decision-to-delivery time between urgency categories during emergency cesarean delivery: a single-center retrospective cohort study. [PDF]
Zhao G +6 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
First clinical implementation of dynamic tumor-tracking volumetric modulated arc therapy with a gimbal-mounted linac: short delivery time and high precision. [PDF]
Kishi N +11 more
europepmc +1 more source
Time of delivery in women with coronavirus disease
Mehdi Rezaee +3 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Spot-optimization reduces beam delivery time in liver breath hold intensity modulated proton therapy. [PDF]
Butkus M +5 more
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source

