Results 51 to 60 of about 4,153 (217)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mechanical characterization of environmentally friendly mixtures

, 2007
Nowadays, road traffic is the main cause of urban pollution. Owing to the primary importance of this problem two different environmentally friendly mixtures were considered: the first to reduce traffic noise and the second to mitigate atmospheric ...
CANESTRARI, FRANCESCO, Canestrari, F, BOCCI, MAURIZIO, FERROTTI, Gilda, Bocci, M., Ferrotti, G., PASQUINI, EMILIANO   +6 more
core  

Study on the Dynamic Properties of the Polyurethane Mixture with Open-Graded Gradation

Polyurethane (PU) mixtures exhibit superior mechanical performance compared to traditional asphalt mixtures, owing to the excellent engineering properties of the PU binder.
Bin Wang, Mingzhu Xu, Shijie Ma, Haisheng Zhao, Peiyu Zhang, Yong Liu, Chunhua Su, Wensheng Zhang   +7 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Evaluation of Tire Rubber-Modified Asphalt Mixtures

, 2021
In the late 1960’s, the use of scrap tire rubber (TR) started in Arizona, USA with the intent to modify the properties of asphalt binders used in paving applications.
Khalil, Stephanos
core   +1 more source

Test Method to Evaluate the Effect of Interface Bond Conditions on Top-Down and Reflective Cracking [PDF]

, 2012
A composite specimen interface-cracking (CSIC) test was developed to evaluate the effect of the characteristics of the interface between asphalt layers on resistance to top-down and reflective cracking.
Lopp, George, Roque, Reynaldo, Chen, Yu
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Laboratory Evaluation of Asphalt Rubber Mixtures Using the Dynamic Modulus (E*)

, 2003
For thirty years, the Arizona Department of Transportation has been adding crumb rubber to the asphalt concrete with remarkable performance. A primary objective of this study was to evaluate the laboratory performance of these mixtures by conducting the ...
Witczak, Matthew, Way, George, Kaloush, Kamil, Sotil Chávez, Andrés   +3 more
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source