Results 171 to 180 of about 4,333,581 (301)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Log-Density Deconvolution by Wavelet Thresholding [PDF]
This paper proposes a new wavelet-based method for deconvolving a density. The estimator combines the ideas of nonlinear wavelet thresholding with periodised Meyer wavelets and estimation by information projection.
Van Bellegem, Sébastien +1 more
core
BACKGROUND: Fractures related to osteoporosis and low bone mineral density lead to substantial morbidity, mortality, and cost to individuals and health systems.
GBD 2021 Low Bone Mineral Density Collaborators
core +1 more source
Human Motion Segmentation via Spatiotemporally Dual-Constrained Density Estimation with Commodity Wi-Fi Device. [PDF]
Sensors (Basel)Wang X, Zhang L, Shu F.
europepmc +1 more source
FEBS Open Bio, EarlyView.The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
Count2Density: Crowd Density Estimation without Location-level Annotations
Crowd density estimation is a well-known computer vision task aimed at estimating the density distribution of people in an image. The primary challenge in this domain is the reliance on fine-grained location-level annotations-i.e., points placed on top ...
Battiato, Sebastiano +5 more
core
Improved density estimation via pair production correction and experimental validation in neutron gamma logging. [PDF]
Sci RepRafizade A, Hosseini SA.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Eye Movement Analysis: A Kernel Density Estimation Approach for Saccade Direction and Amplitude. [PDF]
J Eye Mov ResFehlinger P, Ertl B, Watzka B.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source