Results 1 to 10 of about 26 (26)
Current Protocols, Volume 1, Issue 8, August 2021., 2021 Abstract Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the yeast Saccharomyces cerevisiae.
Natalay Kouprina +2 more
wiley +1 more source
Programmable Gene Knockdown in Diverse Bacteria Using Mobile‐CRISPRi
Current Protocols in Microbiology, Volume 59, Issue 1, December 2020., 2020 Abstract Facile bacterial genome sequencing has unlocked a veritable treasure trove of novel genes awaiting functional exploration. To make the most of this opportunity requires powerful genetic tools that can target all genes in diverse bacteria. CRISPR interference (CRISPRi) is a programmable gene‐knockdown tool that uses an RNA‐protein complex ...
Amy B. Banta +4 more
wiley +1 more source
Molecular Oncology, Volume 14, Issue 11, Page 2920-2935, November 2020., 2020 Liver metastasis is a major cause of colorectal cancer‐related death. Although DNase I displays antimetastatic activity by inhibition of NETs, its clinical use is limited due to its short biologic half‐life. AAV‐mediated gene expression of DNase I is a novel therapeutic strategy that could reduce the development of liver metastases through modulation ...
Yujia Xia +14 more
wiley +1 more source
Current Protocols in Chemical Biology, Volume 11, Issue 3, September 2019., 2019 Abstract Vital cells maintain a steep potassium ion (K+) gradient across the plasma membrane. Intracellular potassium ion concentrations ([K+]) and especially the [K+] within the extracellular matrix are strictly regulated, the latter within a narrow range of ∼3.5 to 5.0 mM.
H. Bischof +6 more
wiley +1 more source
Plant Biotechnology Journal, Volume 16, Issue 1, Page 186-196, January 2018., 2018 Summary Molecular genetic analyses revealed that the WUSCHEL‐related homeobox (WOX) gene superfamily regulates several programs in plant development. Many different mechanisms are reported to underlie these alterations. The WOX family member STENOFOLIA (STF) is involved in leaf expansion in the eudicot Medicago truncutula.
Meiyan Liu +8 more
wiley +1 more source
The Scientific World Journal, Volume 2014, Issue 1, 2014., 2014 To enhance the natural plant resistance and to evaluate the antimicrobial properties of phylloplanin against blue mold, we have expressed a synthetic chimeric native‐phylloplanin‐GFP protein fusion in transgenic Nicotiana tabacum cv. KY14, a cultivar that is highly susceptible to infection by Peronospora tabacina.
Dipak K. Sahoo +6 more
wiley +1 more source
Single Nucleotide Polymorphism Identification, Characterization, and Linkage Mapping in Quinoa
The Plant Genome, Volume 5, Issue 3, November 2012., 2012 Quinoa (Chenopodium quinoa Willd.) is an important seed crop throughout the Andean region of South America. It is important as a regional food security crop for millions of impoverished rural inhabitants of the Andean Altiplano (high plains). Efforts to improve the crop have led to an increased focus on genetic research. We report the identification of
P. J. Maughan +8 more
wiley +1 more source
The spinal muscular atrophy gene product regulates actin dynamics
The FASEB Journal, Volume 38, Issue 18, 30 September 2024.SMN regulates actin dynamics via binding to PFN2a and interacting with F‐ and G‐actin. Thus, two populations, SMN/PFN2a/actin and SMN/actin are relevant for functional actin dynamics. In SMA, SMN protein levels are reduced, PFN2a protein homeostasis becomes dysregulated and F‐actin formation was increased.
Tobias Schüning +8 more
wiley +1 more source
Some of the next articles are maybe not open access.
Deoxyribonuclease is prognostic in patients undergoing transcatheter aortic valve replacement
European Journal of Clinical Investigation, 2021Andreas Mangold +2 more
exaly