Results 11 to 20 of about 32,419 (170)

Highly Selective, CRISPR/Cas9‐Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast

Current Protocols, Volume 1, Issue 8, August 2021., 2021
Abstract Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the yeast Saccharomyces cerevisiae.
Natalay Kouprina, Jung‐Hyun Kim, Vladimir Larionov   +2 more
wiley   +1 more source

Programmable Gene Knockdown in Diverse Bacteria Using Mobile‐CRISPRi

Current Protocols in Microbiology, Volume 59, Issue 1, December 2020., 2020
Abstract Facile bacterial genome sequencing has unlocked a veritable treasure trove of novel genes awaiting functional exploration. To make the most of this opportunity requires powerful genetic tools that can target all genes in diverse bacteria. CRISPR interference (CRISPRi) is a programmable gene‐knockdown tool that uses an RNA‐protein complex ...
Amy B. Banta, Ryan D. Ward, Jennifer S. Tran, Emily E. Bacon, Jason M. Peters   +4 more
wiley   +1 more source

AAV‐mediated gene transfer of DNase I in the liver of mice with colorectal cancer reduces liver metastasis and restores local innate and adaptive immune response

Molecular Oncology, Volume 14, Issue 11, Page 2920-2935, November 2020., 2020
Liver metastasis is a major cause of colorectal cancer‐related death. Although DNase I displays antimetastatic activity by inhibition of NETs, its clinical use is limited due to its short biologic half‐life. AAV‐mediated gene expression of DNase I is a novel therapeutic strategy that could reduce the development of liver metastases through modulation ...
Yujia Xia, Jiayi He, Hongji Zhang, Han Wang, George Tetz, Casey A. Maguire, Yu Wang, Amblessed Onuma, Dmitry Genkin, Victor Tetz, Alexey Stepanov, Stanislav Terekhov, Valeria Ukrainskaya, Hai Huang, Allan Tsung   +14 more
wiley   +1 more source

Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples

Current Protocols in Chemical Biology, Volume 11, Issue 3, September 2019., 2019
Abstract Vital cells maintain a steep potassium ion (K+) gradient across the plasma membrane. Intracellular potassium ion concentrations ([K+]) and especially the [K+] within the extracellular matrix are strictly regulated, the latter within a narrow range of ∼3.5 to 5.0 mM.
H. Bischof, S. Burgstaller, N. Vujic, T. Madl, D. Kratky, W. F. Graier, R. Malli   +6 more
wiley   +1 more source

The STENOFOLIA gene from Medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat

Plant Biotechnology Journal, Volume 16, Issue 1, Page 186-196, January 2018., 2018
Summary Molecular genetic analyses revealed that the WUSCHEL‐related homeobox (WOX) gene superfamily regulates several programs in plant development. Many different mechanisms are reported to underlie these alterations. The WOX family member STENOFOLIA (STF) is involved in leaf expansion in the eudicot Medicago truncutula.
Meiyan Liu, Lei Lei, Fang Miao, Carol Powers, Xiaoyu Zhang, Jungpeng Deng, Million Tadege, Brett F. Carver, Liuling Yan   +8 more
wiley   +1 more source

Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus [PDF]

, 2017
Background: A group A Streptococcus (GAS) lineage of genotype emm3, sequence type 15 (ST15) was associated with a six month upsurge in invasive GAS disease in the UK.
Afshar, B, Al-Shahib, A, Efstratiou, A, Holden, M T G, Lamagni, T L, Smith, K C, Sriskandan, S, Turner, C E, Underwood, A   +8 more
core   +3 more sources

Overexpression of the Synthetic Chimeric Native‐T‐phylloplanin‐GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. tabacum L.)

The Scientific World Journal, Volume 2014, Issue 1, 2014., 2014
To enhance the natural plant resistance and to evaluate the antimicrobial properties of phylloplanin against blue mold, we have expressed a synthetic chimeric native‐phylloplanin‐GFP protein fusion in transgenic Nicotiana tabacum cv. KY14, a cultivar that is highly susceptible to infection by Peronospora tabacina.
Dipak K. Sahoo, Sumita Raha, James T. Hall, Indu B. Maiti, P. Giardina, M. Petruccioli, C. R. C. Calado   +6 more
wiley   +1 more source

Genome characterization and population genetic structure of the zoonotic pathogen, streptococcus canis [PDF]

, 2012
Background - Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen.
Lang, P., Lefébure, T., Moroni, P., Pavinski Bitar, P.D., Richards, V.P., Stanhope, M.J., Tikofsky, L., Werner, B., Zadoks, R.N.   +8 more
core   +3 more sources

Single Nucleotide Polymorphism Identification, Characterization, and Linkage Mapping in Quinoa

The Plant Genome, Volume 5, Issue 3, November 2012., 2012
Quinoa (Chenopodium quinoa Willd.) is an important seed crop throughout the Andean region of South America. It is important as a regional food security crop for millions of impoverished rural inhabitants of the Andean Altiplano (high plains). Efforts to improve the crop have led to an increased focus on genetic research. We report the identification of
P. J. Maughan, S. M. Smith, J. A. Rojas‐Beltrán, D. Elzinga, J. A. Raney, E. N. Jellen, A. Bonifacio, J. A. Udall, D. J. Fairbanks   +8 more
wiley   +1 more source

Successful management of pleural infection with very low dose intrapleural tissue plasminogen activator/deoxyribonuclease regime

Respirology Case Reports, 2019
Pleural infection managed with intrapleural therapy using a combination of 10 mg of tissue plasminogen activator (tPA) and 5 mg of deoxyribonuclease (DNase) has been shown in randomized and open‐label studies to successfully treat >90% of patients ...
Jodi Hart, A. Badiei, Y. C. Lee
semanticscholar   +1 more source