Results 171 to 180 of about 364,005 (184)

Cell-type-specific and site-specific N-glycosylation of type I and type II human tissue plasminogen activator.

Biochemistry, 1989
Tissue plasminogen activator (t-PA) is an important initiator of fibrinolysis. The t-PA polypeptide has four potential N-glycosylation sites of which three are occupied in type I (Asn-117, -184, and -448) and two in type II (Asn-117 and -448).
Raj B. Parekh, Raymond A. Dwek, Jerry R. Thomas, Ghislain Opdenakker, T. W. Rademacher, A. Wittwer, Susan C. Howard, R. Nelson, Ned R. Siegel, M. Jennings, N. K. Harakas, Joseph Feder   +11 more
semanticscholar   +1 more source

Randomized Phase II Trial Comparing Site-Specific Treatment Based on Gene Expression Profiling With Carboplatin and Paclitaxel for Patients With Cancer of Unknown Primary Site.

Journal of Clinical Oncology, 2019
PURPOSE Although gene expression profiling is a promising diagnostic technique to determine the tissue of origin for patients with cancer of unknown primary site (CUP), no clinical trial has evaluated yet site-specific therapy directed by this approach ...
H. Hayashi, T. Kurata, Y. Takiguchi, M. Arai, K. Takeda, K. Akiyoshi, Koji Matsumoto, T. Onoe, H. Mukai, N. Matsubara, H. Minami, M. Toyoda, Y. Onozawa, A. Ono, Y. Fujita, K. Sakai, Y. Koh, A. Takeuchi, Y. Ohashi, K. Nishio, K. Nakagawa   +20 more
semanticscholar   +1 more source

[BME 361 I--a new site-specific deoxyribonuclease type II from Bacillus megaterium 361].

Prikladnaia biokhimiia i mikrobiologiia, 1992
Bme 361 I, a new site-specific type II deoxyribonuclease, was purified from Bacillus megaterium 361 by chromatography on phosphocellulose P 11 and hydroxylapatite. The enzyme recognizes and cleaves the nucleotide sequence 5'-GG decreases CC-3' in double-strand DNA. Thus it is a true isoschizomer of deoxyribonucleases Hae III and BspR I.
L R, Lebedev, I S, Andreeva, G D, Serov, L Ia, Denisova, N M, Pustoshilova   +4 more
openaire   +1 more source

Probing the role of threonine and serine residues of E. coli asparaginase II by site-specific mutagenesis.

Protein Engineering, 1992
Site-specific mutagenesis has been used to probe amino acid residues proposed to be critical in catalysis by Escherichia coli asparaginase II. Thr12 is conserved in all known asparaginases.
C. Derst, J. Henseling, K. Röhm
semanticscholar   +1 more source

Site-specific DNA cleavage by Chlorella virus topoisomerase II.

Biochemistry, 2002
The DNA cleavage reaction of topoisomerase II is central to the catalytic activity of the enzyme and is the target for a number of important anticancer drugs.
J. Fortune, J. Dickey, Oleg V. Lavrukhin, J. V. Van Etten, R. Lloyd, N. Osheroff   +5 more
semanticscholar   +1 more source

Coupled energetics of lambda cro repressor self-assembly and site-specific DNA operator binding II: cooperative interactions of cro dimers.

Journal of Molecular Biology, 2000
The bacteriophage lambda relies on interactions of the cI and cro repressors which self assemble and bind the two operators (O(R) and O(L)) of the phage genome to control the lysogenic to lytic switch.
Paul J. Darling, J. Holt, G. K. Ackers
semanticscholar   +1 more source

Changing the efficiency and specificity of the esterase activity of human carbonic anhydrase II by site-specific mutagenesis.

European Journal of Biochemistry, 1999
Rates of hydrolysis of 4-, 3-, and 2-nitrophenyl acetate and 4-nitrophenyl propionate catalyzed by wild-type and mutant forms of human carbonic anhydrase II have been measured.
B. Elleby, B. Sjöblom, S. Lindskog
semanticscholar   +1 more source

Transforming growth factor-beta receptor type II expression in the hamster ovary: cellular site(s), biochemical properties, and hormonal regulation.

Endocrinology, 1995
The hormonal regulation of transforming growth factor-beta (TGF beta) receptor type II (T beta RII) protein expression in the hamster ovary was evaluated by [125I]TGF beta 1 cross-linking, immunolocalization, and immunoprecipitation of T beta RII using ...
S. K. Roy, A. R. Kole
semanticscholar   +1 more source

An unusual type-II 70-kilodalton keratin protein of mouse epidermis exhibiting postnatal body-site specificity and sensitivity to hyperproliferation.

Differentiation; research in biological diversity, 1987
Keratin extracts from the epidermis of adult mouse ears, footpads, and tail contain large amounts of a 70-kilodalton (kDa) protein which has not been detected in any other body site of the adult mouse or in the epidermis of neonatal mice. Two-dimensional
M. Rentrop, R. Nischt, B. Knapp, J. Schweizer, H. Winter   +4 more
semanticscholar   +1 more source

The amino-terminal cyclic nucleotide binding site of the type II cGMP-dependent protein kinase is essential for full cyclic nucleotide-dependent activation.

Journal of Biological Chemistry, 2000
For the type I cGMP-dependent protein kinases (cGKIalpha and cGKIbeta), a high affinity interaction exists between the C2 amino group of cGMP and the hydroxyl side chain of a threonine conserved in most cGMP binding sites.
Merritt K. Taylor, M. Uhler
semanticscholar   +1 more source