Results 41 to 50 of about 13,337 (203)
Convergent allostery in ribonucleotide reductase
Nature Communications, 2019 Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides, which is an essential step in DNA synthesis. Here the authors use small-angle X-ray scattering, X-ray crystallography, and cryo-electron microscopy to ...
William C. Thomas +7 more
doaj +1 more source
Measurement of FNR-NrdI Interaction by Microscale Thermophoresis (MST)
Bio-Protocol, 2017 This protocol describes how to measure protein-protein interactions by microscale thermophoresis (MST) using the MonolithTM NT.115 instrument (NanoTemper).
Ingvild Gudim +3 more
doaj +1 more source
Deoxyribonucleotide Biosynthesis in Synchronous Algae Cells [PDF]
European Journal of Biochemistry, 1980 Synchronous cells of the green alga, Scenedesmus obliquus, cultured in a 14‐h/10‐h light/dark regime, contain a peak of ribonucleoside‐diphosphate reductase activity and maximum deoxyribonucleoside 5′‐triphosphate concentrations at the 12th hour of the cell cycle, coinciding with DNA synthesis and preceding the formation of eight daughter cells.
W, Feller +2 more
openaire +2 more sources
Cells, 2021 Nucleotides fulfill many essential functions in plants. Compared to non-plant systems, these hydrophilic metabolites have not been adequately investigated in plants, especially the less abundant nucleotide species such as deoxyribonucleotides and ...
Henryk Straube +2 more
doaj +1 more source
Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone [PDF]
, 1975 The mouse thymus contains two forms of terminal deoxynucleotidyl transferase (TdT) which are distinguishable by the salt concentration necessary to elute them from a phosphocellulose column, by their distrubtion among the thymocyte subpopulations, and by
Baltimore, David +3 more
core +1 more source
The Importance of Mitochondrial DNA in Aging and Cancer
Journal of Aging Research, 2011 Mitochondrial dysfunction has been implicated in premature aging, age-related diseases, and tumor initiation and progression. Alterations of the mitochondrial genome accumulate both in aging tissue and tumors.
Claus Desler +3 more
doaj +1 more source
Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]
, 2020 The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Aldave, Anthony J +14 more
core
Conventional Raman, SERS and TERS Studies of DNA Compounds
Chemosensors, 2023 DNA identification is possible by detecting its components through vibrational spectroscopy. Conventional Raman, Surface-enhanced Raman spectroscopy (SERS) and Tip-enhanced Raman spectroscopy (TERS) have shown a high capacity for the exploration of ...
Wafa Safar +3 more
doaj +1 more source
Ubiquitination and deubiquitination of PCNA in response to stalling of the replication fork [PDF]
, 2009 Following exposure of human cells to DNA damaging agents that block the progress of the replication fork, mono-ubiquitination of PCNA mediates the switch from replicative DNA polymerases to polymerases specialised for translesion synthesis. We have shown
Brown, Stephanie +2 more
core +2 more sources
Efficient Enantiomeric Selectivity Separation Via Homochiral Graphene Oxide Membrane
ENERGY &ENVIRONMENTAL MATERIALS, EarlyView.A new type of chiral graphene oxide (GO) membrane is proposed that exhibits a separation factor of up to 20.1 as well as a preferential enantiomer flux of 4.5 mmol m−2 h−1. Moreover, the only first FPLC‐compatible column has been further developed via stacking these chiral GO membranes.
Junbo Wang +10 more
wiley +1 more source