Results 141 to 150 of about 10,071,563 (340)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Mid-level Deep Pattern Mining∗

, 2015
Mid-level visual element discovery aims to find clusters of image patches that are both representative and discrimi-native. In this work, we study this problem from the prospec-tive of pattern mining while relying on the recently popular-ized ...
Anton Van Den Hengel, Lingqiao Liu, Van Den Hengel, A., Liu, L., Shen, C., Chunhua Shen, Li, Y., Yao Li   +7 more
core   +1 more source

OPTIMIZATION OF FDM 3D PRINTING PARAMETERS ON THE TENSILE STRENGTH OF FOOD-GRADE PLA FILAMENT USING THE TAGUCHI METHOD

Rekayasa Mesin
Additive manufacturing, particularly 3D printing, has emerged as a transformative technology in digital fabrication, enabling the rapid, accurate, and cost-effective production of complex components.
Pristiansyah Pristiansyah, Hasdiansah Hasdiansah, Muhammad Subhan, Sukanto Sukanto, Fajar Aswin, Masdani Masdani, Ariansyah Ariansyah   +6 more
doaj   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

PATTERNS OF FIBRIN DEPOSITION IN THE GLOMERULI OF DISEASED KIDNEYS

Japanese Circulation Journal, 1976
Immunofluorescent studies were performed on 217 percutaneous renal biopsies on patients with various renal diseases, which were examined in detail to assess the amount, character, and distribution of fibrin deposits in the glomeruli. The fibrin deposits were classified into six different forms on immunohistologic grounds.
openaire   +3 more sources

Polymorphisms in SAA alter intrarenal amyloid distribution of AA amyloidosis in cats

Scientific Reports
The kidneys are one of the primary organs affected by amyloid A (AA) amyloidosis in mixed-breed cats. The distribution of amyloid deposits within the kidneys varies among individuals; however, the underlying cause is unknown.
Natsumi Kobayashi, Masahiro Kaneda, Mitsuhiro Ikeda, Hirotaka Kondo, Susumu Iwaide, Yoshiyuki Itoh, Miki Hisada, Yuka Kato, Niki Sedghi Masoud, Kohji Nomura, Machie Tsuneyasu, Tomoko Akamine, Tomoaki Murakami   +12 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source