Results 151 to 160 of about 3,113 (211)
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
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Descriptive Norms and Norm Innovations
Journal of Cross-Cultural Psychology, 2015Morris and Liu’s (2015) article about norm innovation in the special issue of the Journal of Cross-Cultural Psychology (JCCP) about descriptive norms adds theoretical substance (Leung, 1989; Peterson & Barreto, 2014; Triandis & Suh, 2002) to studies that statistically compare measures at different levels of analysis(e.g., Fischer, Vauclair, Fontaine,
Peterson, Mark F., Barreto, Tais S.
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The Normative and the Descriptive
2021Abstract This chapter introduces some of the basic themes of Causation with a Human Face. Accounts of causal reasoning can either be normative (concerned with how we ought to reason) or descriptive (concerned with how we in fact reason).
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Conformity to descriptive norms
Journal of Economic Behavior & Organization, 2022Descriptive norms, which measure the prevalence of prosocial behavior (prosocial “contribution”, in our context), powerfully influence individual prosocial choice. We investigate the relative importance of three channels through which this may occur: (1) Individuals directly prefer to conform to common behavior, (2) individuals reciprocally contribute ...
Vera L. te Velde, Winnifred Louis
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Decision Analysis, 2008
This article addresses the problem of identifying conditions according to which it is possible to distinguish between a descriptive theory and a normative theory. What makes a descriptive theory descriptive and a normative theory normative? The focus is on subjective expected utility theories where it seems open to debate whether the appropriate use is
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This article addresses the problem of identifying conditions according to which it is possible to distinguish between a descriptive theory and a normative theory. What makes a descriptive theory descriptive and a normative theory normative? The focus is on subjective expected utility theories where it seems open to debate whether the appropriate use is
openaire +1 more source
Descriptions, Prescriptions and Norms
2022In his Scope and Method of Economics (1890), John Neville Keynes applied a tripartite distinction to the tasks of economists, dividing these into descriptions, norms and prescriptions. Keynes’s book both recapitulated debates revolving around the goals and limits of Classical political economy and opened a path towards debates between contemporary ...
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Unpacking Normativity. Conceptual, Normative, and Descriptive Issues
Jurisprudence, 2021The anthology Unpacking Normativity, edited by Kenneth Einar Himma, Miodrag Jovanovic, and Bojan Spaic, follows the international conference ‘Legal Normativity and Language’ which took place at the...
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