Results 81 to 90 of about 1,705,946 (235)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Reflecting on Increasing Inclusivity through Distributed Co-Design Over Two Decades

Interaction Design and Architecture(s)
Co-design, an integral practice in design research, has evolved significantly in recent years. This paper offers a practice-led reflection of distributed co-design through two decades of intergenerational projects, from early challenges to its ...
Greg Walsh
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Working Right Ways in Foot Health With and for First Nations Peoples: Research Method Guided and Governed by First Nations Ways of Knowing, Being, and Doing in Cross‐Sectional Qualitative Study Design

Journal of Foot and Ankle Research
Background Underpinning ongoing colonisation of the lands now known as Australia, scientific racism in colonial research delivered flawed results by building Indigenous inferiority into methodology to produce dehumanising conclusions of First Nations ...
James Gerrard, Shirley Godwin (Badimaya Yamatji), Kim Whiteley (Wiradjuri), James Charles (Kaurna), Sean Sadler, Vivienne Chuter   +5 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Design Thinking, Acting, and Making Net Zero Transformational Change Across NHS Scotland

Societies
Climate change is the biggest global health threat of the 21st century. However, this challenge presents an opportunity to do things differently. This paper sets out how, using a design-led and collaborative approach, one can re-imagine the delivery of ...
Paul A. Rodgers, Mel Woods, Sonja Oliveira, Efstathios Tapinos, David Bucknall, Fraser Bruce, Andrew Wodehouse, Gregor White, Marc P. Y. Desmulliez   +8 more
doaj   +1 more source

Living together in old age: Identifying preferences for collaborative housing through participatory design-led research

Berliner Journal für Soziologie
Abstract In the Netherlands, as in many European countries, housing has become increasingly commodified, and there are growing disparities amongst the housing situations of different generations. While older people tend to live in spacious homes, young people and families increasingly struggle to find a suitable dwelling. Furthermore,
openaire   +1 more source