Results 51 to 60 of about 3,339,955 (267)
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Design of Cascaded DOEs for Focusing Different Wavelengths to Different Points
PhotonicsWe propose a task-specific method for calculating cascaded phase diffractive optical elements (DOEs) for focusing Q incident beams with different wavelengths to Q given points.
Leonid L. Doskolovich +6 more
doaj +1 more source
Constructive Methods of Program Design [PDF]
, 1976 Correct programs cannot be obtained by attempts to test or to prove incorrect programs: the correctness of a program should be assured by the design procedure used to build it.
openaire +1 more source
Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?
FEBS Letters, EarlyView.This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes +3 more
wiley +1 more source
Applied Sciences, 2017 The output and input coupling characteristics of the compliant parallel mechanism (CPM) bring difficulty in the motion control and challenge its high performance and operational safety.
Yao Jiang +3 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
BuildingsSteel columns, which are widely used in building frameworks and spatial structures, are susceptible to capacity degradation during fires, potentially leading to the overall collapse of buildings.
Xiaoming Wang +3 more
doaj +1 more source
The True Benefits of Designing Design Methods
Artifact, 2014 Abstract This paper calls for a new way of understanding and using methods in human-centred design. Design researchers have recently been active in developing new types of methods aimed at greatly improving their empathic understanding of people’s holistic experience, and their design imagination.
openaire +6 more sources
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source