Results 141 to 150 of about 1,282,652 (268)

Design and experiment of a comb-and-scrape Idesia Polycarpa Maxim threshing device. [PDF]

Sci Rep
Yang G, Wu D, Xu W, Tang Y.
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Design and experiment of a novel intelligent device suitable for automation vegetable plug seedling. [PDF]

PLoS One
Haifei Q, Zhike N, Xiandong C, Bo L, Yifan Z, Feiyang L, Jiajun Z.   +6 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Optimized design and experiment of a controllable rotary tube strip fertilizer spreader for hilly mountainous terrain. [PDF]

Sci Rep
Dun G, Gao S, Ji X, Sun H, Wei Y, Sheng Q, Zhang C, Ma C, Li X, Wang L, Yu Z, Li H, Ma C.   +12 more
europepmc   +1 more source

Design of Experiment for Optimizing Microencapsulation by the Solvent Evaporation Technique. [PDF]

Polymers (Basel), 2023
Loureiro MV, Aguiar A, Santos RGD, Bordado JC, Pinho I, Marques AC.   +5 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity. [PDF]

Vaccines (Basel)
Hu C, Wang H, Liu G, Li K, Zhang X, Song L, Gao F, Wu X, Wang Q, Liu M, Liu J, Fu Z, Ma X, Xu M, Mao Q, Liang Z, He Q.   +16 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Design and Experiment of an Automatic Leveling System for Tractor-Mounted Implements. [PDF]

Sensors (Basel)
Yao H, Zhang E, Liu Y, Du J, Yin X.
europepmc   +1 more source