Results 221 to 230 of about 4,561,954 (333)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments. [PDF]

Biotechnol Prog
Aw R, Polizzi K.
europepmc   +1 more source

The optimal experiment? Influence of solution strategies on model-based optimal experimental design

Volodymyr Kozachynskyi, Markus Illner, Erik Esche, Jens‐Uwe Repke   +3 more
openalex   +1 more source

Privacy by Design in Machine Learning Data Collection: An Experiment on Enhancing User Experience [PDF]

Yang Xu, Yingchia Liu, Jiang Wu, Xiaoan Zhan   +3 more
openalex   +1 more source

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

FEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner, César G. Prucca, Lucia Boffelli, Virginia A. Piqueras, Silvia G. Correa, Mariana Maccioni, Mario E. Guido   +6 more
wiley   +1 more source

Effective Design for Experiments on Small-Group Conversation: Insights From an Example Study. [PDF]

Am J Audiol
Nicoras R, Buck B, Fischer RL, Godfrey M, Hadley LV, Smeds K, Naylor G.   +6 more
europepmc   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Encapsulation of Acid Whey in Alginate Microspheres for Application in Skin Microbiome-Friendly Topical Formulations: Optimization Through a Design of Experiments Approach. [PDF]

Molecules
Sikora E, Łętocha A, Michalczyk A, Kozik A.   +3 more
europepmc   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Optimization of Enterovirus-like Particle Production and Purification Using Design of Experiments. [PDF]

Pathogens
Kuijpers L, van den Braak WJP, Freydoonian A, Dekker NH, van der Pol LA.   +4 more
europepmc   +1 more source