Results 151 to 160 of about 1,098,050 (261)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Expanding the Design Space for Fall Prevention in Acute Orthopedic Hospital Care: Human-Centered Design Study. [PDF]

JMIR Hum Factors
Ehn M, Revenäs Å, Tobiasson H.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Determination and validation of design space for mesenchymal stem cell cultivation processes using prediction intervals. [PDF]

Commun Biol
Hirono K, Hayashi Y, A Udugama I, Gaddem MR, Tanaka K, Takemoto Y, Kato R, Kino-Oka M, Sugiyama H.   +8 more
europepmc   +1 more source

New operations for truncus arteriosus repair using partial heart transplantation: Exploring the surgical design space with 3-dimensional printed heart models. [PDF]

JTCVS Tech, 2023
Rajab TK, Kang L, Hayden K, Andersen ND, Turek JW.   +4 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

A Mathematical Model for Determining Probabilistic Design Space in Mesenchymal Stem Cell Passage Culture. [PDF]

Biotechnol Bioeng
Hirono K, Hayashi Y, Ogawa Y, Kino-Oka M, Sugiyama H.   +4 more
europepmc   +1 more source

Expanding Biomaterial Surface Topographical Design Space through Natural Surface Reproduction. [PDF]

Adv Mater, 2021
Vermeulen S, Honig F, Vasilevich A, Roumans N, Romero M, Dede Eren A, Tuvshindorj U, Alexander M, Carlier A, Williams P, Uquillas J, de Boer J.   +11 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

The design space of E(3)-equivariant atom-centred interatomic potentials. [PDF]

Nat Mach Intell
Batatia I, Batzner S, Kovács DP, Musaelian A, Simm GNC, Drautz R, Ortner C, Kozinsky B, Csányi G.   +8 more
europepmc   +1 more source