Results 181 to 190 of about 93,770 (286)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A multidisciplinary experimental and methodological investigation of electron and proton minibeams in the framework of the INFN MIRO project. [PDF]

Med Phys
Romano F, Zanacchi FC, Ciarrocchi E, Franciosini G, Milluzzo G, Scifoni E, Vignati A, Ahmad S, Arezzini S, Attili A, Battestini M, Bernardini J, Bettanin M, Bisio A, Bisogni MG, Bodrenko I, Bordieri G, Bravatà V, Burattini A, Camarda M, Cammarata FP, Capaccioli S, Castelli L, Cavalieri A, Celentano M, Chiadroni E, Cordoni FG, Corvaia E, Costa M, Curcio A, Da Pozzo E, De Felice M, Debbio FD, D'Errico F, D'Oca MC, D'Orsi B, Deut U, Durisi E, Fantacci ME, Farina S, Felici G, Ferro A, Ficcadenti L, Formuso A, Forte GI, Giordanengo S, Giuliano A, Giuliano L, Lanzanò L, Linsalata S, Lossano S, Maffei M, Marafini M, Marrale M, Massa M, Masturzo L, Mazzoni E, Migliorati M, Milian FM, Minafra L, Moggi A, Olivares DM, Montefiori M, Morrocchi M, Mostacci A, Mostardi F, Okpuwe C, Paiar F, Palumbo L, Patera V, Pensavalle JH, Pucci G, Quattrini F, Retico A, Rosso V, Russo G, Sacchi R, Sarti A, Scalisi S, Scapicchio C, Schiavi A, Stochino P, Strettoi E, Tommasino F, Tozzini V, Traini G, Trovato G, Usai A, Urso F, Martino FD.   +89 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Experimental activation assessment of open-type yoke in a PET cyclotron by non-destructive/destructive γ-ray measurement approaches. [PDF]

Med Phys
Yoshida G, Matsumura H, Toyoda A, Nakamura H, Masumoto K, Miura T, Watanabe E, Tsugane K, Saito K, Koumura I, Uno H, Kozaki M, Yamamoto A, Toyohara J, Wagatsuma K.   +14 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dual X-Ray and Optical System for In Situ Monitoring of Solids Fraction in Tailings. [PDF]

Sensors (Basel)
Sraw TK, Yu B, Liu X, Ng J, Sedgwick A, Gupta M, Fedosejevs R, Tsui YY.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Generalized self-calibrating probe approach for CW tissue oximetry. [PDF]

J Biomed Opt
Motamed Jahromi L, Yang L, von Lühmann A, Grosenick D.   +3 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

One million scans later: the evolution of the I16 material and magnetism beamline at Diamond Light Source. [PDF]

J Synchrotron Radiat
Abdeldaim AH, Scatena R, Hawkins J, Nisbet G, Porter DG, Barlow G, Marshall A, Nutter B, Beutier G, Collins SP, Bombardi A.   +10 more
europepmc   +1 more source