Results 181 to 190 of about 5,957,804 (303)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Development of Novel Surface-Enhanced Raman Spectroscopy-Based Biosensors by Controlling the Roughness of Gold/Alumina Platforms for Highly Sensitive Detection of Pyocyanin Secreted from Pseudomonas aeruginosa. [PDF]

Biosensors (Basel)
El-Said WA, Saleh TS, Al-Bogami AS, Wani MY, Choi JW.   +4 more
europepmc   +1 more source

Development of a surface tension mediated technique for dry stabilization of mammalian cells. [PDF]

PLoS One, 2018
Solocinski J, Osgood QA, Rosiek E, Underwood L, Zikanov O, Chakraborty N.   +5 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

The Development of a Surface Finisher of Car Park Slab Using Waterborne Silicon Acrylic with Polyamide [Part II: Safety Tests]. [PDF]

Materials (Basel), 2019
Gong J, Jeong H, Cho D.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The Development of a Surface Finisher of Car Park Slab Using Waterborne Silicon Acrylic with Polyamide [Part I: Performance Evaluation]. [PDF]

Materials (Basel), 2019
Jeong H, Gong J, Yoon W, Cho D.
europepmc   +1 more source

The development of the surface mesh quality improvement

The development of the surface mesh quality improvement
identifier:oai:t2r2.star.titech.ac.jp ...
openaire  

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Development of response surface methodology for optimization of parameters and quantitative analysis of chebulinic acid from composition of medicinal herbs by HPLC. [PDF]

Saudi J Biol Sci, 2019
Munawar TM, Surya Prakash DV, Vangalapati M.   +2 more
europepmc   +1 more source