Sequencing and expression of the aroA gene from Dichelobacter nodosus
Gene, 1994The aroA locus of the Gram- pathogen Dichelobacter nodosus, which encodes 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, has been sequenced and expressed in Escherichia coli. The gene is located on a 1.48-kb DraI-HindIII fragment located directly upstream and in opposite transcriptional orientation to the gene encoding the fimbrial structural ...
Alm, Richard A. +2 more
openaire +3 more sources
Dichelobacter nodosus serotype M fimbrial subunit gene: implications for serological classification
Veterinary Microbiology, 2001Dichelobacter nodosus fimbrial subunit gene (fimA) from a serotype M strain (M-SPAHL) was investigated in this study. A primer set targeting the relatively conserved fimA regions and based on the published sequence from Nepalese serogroup M isolates (Nepalese M), failed to amplify the fimA of M-SPAHL. However, when the downstream primer was substituted
H, Zhou, J G, Hickford
openaire +2 more sources
The laboratory culture of Dichelobacter nodosus in a footrot eradication program
Australian Veterinary Journal, 1994As part of a program to eradicate virulent footrot from Western Australia, 2745 isolates of Dichelobacter nodosus were isolated from 5263 specimens from 1883 submissions. The virulence of each isolate was assessed using protease thermostability and isoenzyme zymogram.
D R, Pitman, M A, Palmer, L J, Depiazzi
openaire +2 more sources
Molecular biology of surface antigens of Dichelobacter nodosus
2021This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
openaire +1 more source
Molecular analysis of the rRNA genes of Dichelobacter nodosus
2021This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
openaire +1 more source
Identification of two new serotypes within serogroup B of Dichelobacter nodosus
Anaerobe, 2012The present study records the strain-specific molecular typing system for Dichelobacter nodosus (D. nodosus) based on genetic analysis of fimA locus. Based on the study two new serotypes B5 and B6 are reported within the serogroup B. Out of 200 swab samples collected randomly from foot lesions of footrot affected sheep from all the districts of Kashmir,
M A, Bhat +4 more
openaire +2 more sources
Detection of Dichelobacter nodosus using species-specific oligonucleotides as PCR primers
Veterinary Microbiology, 1993Dichelobacter nodosus is an essential causative agent of ovine footrot, a disease of major economic significance. Four oligonucleotides complementary to variable regions of the 16S rRNA of D. nodosus were identified, synthesized and tested for their specificity and sensitivity as probes for the detection of D. nodosus.
S, La Fontaine, J R, Egerton, J I, Rood
openaire +2 more sources
Fusobacterium necrophorum, and not Dichelobacter nodosus, is associated with equine hoof thrush
Veterinary Microbiology, 2013The aim of this study was to determine which of the two species, Fusobacterium necrophorum or Dichelobacter nodosus, are associated with hoof thrush in horses. Fourteen hoof samples, collected from eight horses with thrush and 14 samples collected from eight horses with healthy hooves, were examined for the presence of F.
Kaloyan K, Petrov, Leon M T, Dicks
openaire +2 more sources
Expression in Escherichia coli of the extracellular basic protease from Dichelobacter nodosus
Microbiology, 1994Dichelobacter nodosus, a Gram-negative obligate anaerobe and the causative agent of ovine footrot, secretes a number of extracellular proteases, one of which is highly basic in nature. The gene (bprV) encoding this basic protease, from virulent strain 198, has been cloned and sequenced. Clone pBR3KB contained the complete bprV gene which constitutively
P R, Vaughan +4 more
openaire +2 more sources
Physical and genetic map of the chromosome of Dichelobacter nodosus strain A198
Gene, 1997A physical map of the chromosome of Dichelobacter nodosus strain A198 was constructed using the restriction endonucleases EagI and StuI. Mapping data indicated the presence of a single, circular chromosome of 1.54 Mb. The three rRNA operons and the virulence related locus (vrl) were precisely positioned at the junctions of EagI and StuI fragments, and ...
S, La Fontaine, J I, Rood
openaire +2 more sources

