Results 261 to 270 of about 233,078 (308)
Some of the next articles are maybe not open access.
Understanding Restriction Enzyme Digests
2021Prior to submitting genomic DNA for sequencing, SEA students perform a restriction endonuclease digest with several enzymes, which provides a fast and cost-effective way of quickly screening isolated phages. The pattern of bands on the gel should be unique except for very closely related or identical phages, which is useful when trying to identify ...
Agrawal, Swati +4 more
openaire +1 more source
Transpancreatic transport of digestive enzyme
Biochimica et Biophysica Acta (BBA) - General Subjects, 1979When porcine alpha-amylase or bovine chymotrypsinogen A was added to the medium bathing the rabbit pancreas in short-term organ culture, the secretion of these enzymes collected via the duct system increased greatly. To determine if it was indeed the amylase added to the bath that was recovered in secretion, endogenous enzyme stores were prelabeled ...
L D, Isenman, S S, Rothman
openaire +2 more sources
Proteolytic Digestion Rates for Altered Enzymes
Radiation Research, 1970Native and unfolded forms of ribonuclease, lysozyme, and trypsin have been subjected to partial proteolysis with trypsin or pronase. Soluble peptides are formed from the mixed disulfides of lysozyme or ribonuclease, with cystine at a rate intermediate between the rates of soluble peptide formation from native and other unfolded forms of these two ...
C O, Stevens, H E, Sauberlich
openaire +2 more sources
Pancreatic Enzyme Products: Digesting the Changes
Annals of Pharmacotherapy, 2011Objective: To review the pharmacology, dosage regimens, efficacy, and safety of currently marketed pancreatic enzyme products (PEPs). Data Sources: Studies were identified by PubMed (1966-January 2011), clintcaltrials.gov, fda.gov, and International Pharmaceutical Abstracts. Search terms included pancreatic enzyme, lipase, Creon, Zenpep, Pancreaze, and
Christopher A, Giuliano +2 more
openaire +2 more sources
Fasciola hepatica: A proteolytic digestive enzyme
Experimental Parasitology, 1980Abstract A proteolytic enzyme in the gut exudate of the common liver fluke has been purified. The enzyme is specific for globin with a pH optimum of 3.9–4.0 and breaks the protein down to peptides and a small percentage of free amino acids. Collagenase activity at pH 7.5 copurifies with the main globinolytic enzyme.
K G, Simpkin, C R, Chapman, G C, Coles
openaire +2 more sources
COMPLETE ENZYMIC DIGESTION OF ACIDIC PROTEINS
International Journal of Peptide and Protein Research, 1976Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of “unusual” amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins.The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino‐peptidase plus prolidase ...
openaire +2 more sources
Carbohydrate-active enzymes (CAZymes) in the gut microbiome
Nature Reviews Microbiology, 2022Jacob F Wardman +2 more
exaly
Structural biology of CRISPR–Cas immunity and genome editing enzymes
Nature Reviews Microbiology, 2022Joy Y Wang +2 more
exaly