Low-intensity illumination for lensless digital holographic microscopy with minimized sample interaction. [PDF]
Exposure to laser light alters cell culture examination via optical microscopic imaging techniques, also based on label-free coherent digital holography. To mitigate this detrimental feature, researchers tend to use a broader spectrum and lower intensity of illumination, which can decrease the quality of holographic imaging due to lower resolution and ...
Mirecki B+6 more
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Imaging in digital holographic microscopy
We present a theoretical formalism for three dimensional (3D) imaging properties of digital holographic microscopy (DHM). Through frequency analysis and visualization of its 3D optical transfer function, an assessment of the imaging behavior of DHM is given. The results are compared with those from other types of interference microscopy.
Shan Shan Kou, Colin J. R. Sheppard
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Lipid quantification in living microalgal cultures with digital holographic microscopy [PDF]
Large amounts of lipids are stored inside lipid droplets by some microalgae. Since these lipids can be used to produce nutraceuticals and biodiesel in a sustainable way, research is developing on fast non-destructive methods to quantify and monitor the ...
Catherine Yourassowsky+3 more
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Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy [PDF]
Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a ...
Lucia Gigli+4 more
doaj +2 more sources
Human live spermatozoa morphology assessment using digital holographic microscopy [PDF]
Digital holographic microscopy (DHM) was applied for the morphological assessment of live intact spermatozoa from fertile and infertile men directly after semen liquefaction.
Marzena Kamieniczna+4 more
doaj +2 more sources
pyDHM: A Python library for applications in digital holographic microscopy. [PDF]
pyDHM is an open-source Python library aimed at Digital Holographic Microscopy (DHM) applications. The pyDHM is a user-friendly library written in the robust programming language of Python that provides a set of numerical processing algorithms for ...
Raul Castañeda+2 more
doaj +2 more sources
Grading of glioma tumors using digital holographic microscopy [PDF]
Gliomas are the most common type of cerebral tumors; they occur with increasing incidence in the last decade and have a high rate of mortality. For efficient treatment, fast accurate diagnostic and grading of tumors are imperative. Presently, the grading
Violeta L. Calin+11 more
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Principles and techniques of digital holographic microscopy [PDF]
Digital holography is an emerging field of new paradigm in general imaging applications. We present a review of a subset of the research and development activities in digital holography, with emphasis on microscopy techniques and applications. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are ...
Myung K. Kim
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Deep learning with digital holographic microscopy discriminates apoptosis and necroptosis [PDF]
Regulated cell death modalities such as apoptosis and necroptosis play an important role in regulating different cellular processes. Currently, regulated cell death is identified using the golden standard techniques such as fluorescence microscopy and ...
Joost Verduijn+3 more
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Tip Crack Imaging on Transparent Materials by Digital Holographic Microscopy [PDF]
With this study, we propose a method to image the tip crack on transparent materials by using digital holographic microscopy. More specifically, an optical system based on Mach−Zehnder interference along with an inverted microscopy (Olympus CKX53 ...
Wen-Jing Zhou+6 more
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