Results 1 to 10 of about 43,419 (242)

Application of Digital Holographic Microscopy to Analyze Changes in T-Cell Morphology in Response to Bacterial Challenge. [PDF]

Cells, 2023
Quantitative phase imaging (QPI) is a non-invasive, label-free technique used to detect aberrant cell morphologies caused by disease, thus providing a useful diagnostic approach.
Vom Werth KL, Kemper B, Kampmeier S, Mellmann A.   +3 more
europepmc   +2 more sources

pyDHM: A Python library for applications in digital holographic microscopy. [PDF]

PLoS One, 2022
pyDHM is an open-source Python library aimed at Digital Holographic Microscopy (DHM) applications. The pyDHM is a user-friendly library written in the robust programming language of Python that provides a set of numerical processing algorithms for ...
Castañeda R, Trujillo C, Doblas A.
europepmc   +2 more sources

Low-intensity illumination for lensless digital holographic microscopy with minimized sample interaction. [PDF]

Biomed Opt Express, 2022
Exposure to laser light alters cell culture examination via optical microscopic imaging techniques based on label-free coherent digital holography. To mitigate this detrimental feature, researchers tend to use a broader spectrum and lower intensity of ...
Mirecki B, Rogalski M, Arcab P, Rogujski P, Stanaszek L, Józwik M, Trusiak M.   +6 more
europepmc   +3 more sources

Label-Free Digital Holographic Microscopy for In Vitro Cytotoxic Effect Quantification of Organic Nanoparticles. [PDF]

Cells, 2022
Cytotoxicity quantification of nanoparticles is commonly performed by biochemical assays to evaluate their biocompatibility and safety. We explored quantitative phase imaging (QPI) with digital holographic microscopy (DHM) as a time-resolved in vitro ...
Eder KM, Marzi A, Barroso Á, Ketelhut S, Kemper B, Schnekenburger J.   +5 more
europepmc   +2 more sources

Real-time 3D tracking of swimming microbes using digital holographic microscopy and deep learning. [PDF]

PLoS One
The three-dimensional swimming tracks of motile microorganisms can be used to identify their species, which holds promise for the rapid identification of bacterial pathogens.
Matthews SA, Coelho C, Rodriguez Salas EE, Brock EE, Hodge VJ, Walker JA, Wilson LG.   +6 more
europepmc   +2 more sources

COVID-19 screening with digital holographic microscopy using intra-patient probability functions of spatio-temporal bio-optical attributes. [PDF]

Biomed Opt Express, 2022
We present an automated method for COVID-19 screening using the intra-patient population distributions of bio-optical attributes extracted from digital holographic microscopy reconstructed red blood cells.
O'Connor T, Javidi B.
europepmc   +2 more sources

Digital Holographic Microscopy for Label-Free Detection of Leukocyte Alternations Associated with Perioperative Inflammation after Cardiac Surgery. [PDF]

Cells, 2022
In a prospective observational pilot study on patients undergoing elective cardiac surgery with cardiopulmonary bypass, we evaluated label-free quantitative phase imaging (QPI) with digital holographic microscopy (DHM) to describe perioperative ...
Steike DR, Hessler M, Korsching E, Lehmann F, Schmidt C, Ertmer C, Schnekenburger J, Eich HT, Kemper B, Greve B.   +9 more
europepmc   +2 more sources

Image Processing Techniques for Improving Quality of 3D Profile in Digital Holographic Microscopy Using Deep Learning Algorithm. [PDF]

Sensors (Basel)
Digital Holographic Microscopy (DHM) is a 3D imaging technology widely applied in biology, microelectronics, and medical research. However, the noise generated during the 3D imaging process can affect the accuracy of medical diagnoses.
Kim HW, Cho M, Lee MC.
europepmc   +2 more sources

Noise Filtering Method of Digital Holographic Microscopy for Obtaining an Accurate Three-Dimensional Profile of Object Using a Windowed Sideband Array (WiSA). [PDF]

Sensors (Basel), 2022
In the image processing method of digital holographic microscopy (DHM), we can obtain a phase information of an object by windowing a sideband in Fourier domain and taking inverse Fourier transform.
Kim HW, Cho M, Lee MC.
europepmc   +2 more sources

Deep learning with digital holographic microscopy discriminates apoptosis and necroptosis. [PDF]

Cell Death Discov, 2021
Regulated cell death modalities such as apoptosis and necroptosis play an important role in regulating different cellular processes. Currently, regulated cell death is identified using the golden standard techniques such as fluorescence microscopy and ...
Verduijn J, Van der Meeren L, Krysko DV, Skirtach AG.   +3 more
europepmc   +2 more sources