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Superesolution in digital holographic microscopy
2011 10th Euro-American Workshop on Information Optics, 2011In this contribution, we address with the possibility to overcome the limited resolving power of imaging systems beyond the limit imposed by Abbe's diffraction theory. We first review the mathematical foundations underlying superresolution (SR) from an information theory point of view and then we focus on two multiplexing approaches in digital ...
Alejandro Calabuig +4 more
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Digital Holographic Microscopy (DHM)
Imaging & Microscopy, 2006Digital Holographic Microscopes (DHM) enables strictly noninvasive visualisation of unstained transparent, and partially reflective specimens, in real time, by providing simultaneously amplitude and phase changes of, a light wave transmitted or reflected.
Yves Emery +5 more
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Digital Holographic Microscopy to Assess Cell Behavior
2023Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality checks, selection and setup of imaging chambers, a ...
Brad, Bazow +5 more
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Digital Holography and Digital Holographic Microscopy
2015Whereas conventional analog holography (AH) entails physical re-illumination of the hologram of the object with a reading beam and finding the 3D real or virtual image, digital holography (DH) is a numerical procedure that simulates Fresnel diffraction of light from the re-illuminated hologram on a computer and numerically determines the images, which ...
Georges T. Nehmetallah +2 more
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Autofocusing in digital holographic microscopy
3D Research, 2011Many applications in non-destructive testing at a microscopic level and in live cell imaging require automated focusing due to unstable environmental conditions, moving specimen or the limited depth of field of the applied optical imaging systems.
Patrik Langehanenberg +2 more
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Coherence effects in digital in-line holographic microscopy
Journal of the Optical Society of America A, 2008We analyze the effects of partial coherence in the image formation of a digital in-line holographic microscope (DIHM). The impulse response is described as a function of cross-spectral density of the light used in the space-frequency domain. Numerical simulation based on the applied model shows that a reduction in coherence of light leads to broadening
Unnikrishnan, Gopinathan +2 more
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Tomography by point source digital holographic microscopy
Applied Optics, 2014We propose a tomographic method for point source inline holographic microscopy. By recording a set of holograms at different illumination angles, shadowing effects are eliminated resulting in three-dimensional images with the same precision at the micrometer-scale in all directions.
Clément, Remacha +2 more
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Simultaneous Multiplane Imaging in Digital Holographic Microscopy
Journal of Display Technology, 2011We describe a numerical method applied to the reconstruction of a digital hologram, that allows to obtain the image of an object at three planes at different depths simultaneously. In order to do this, we introduce a numerical quadratic deformed diffraction grating into the algorithm.
Paturzo Melania +2 more
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Cellular Dynamics Revealed by Digital Holographic Microscopy☆
2017Digital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase
Marquet, P. +2 more
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Digital Holographic Microscopy
Imaging & Microscopy, 2009Björn Kemper +4 more
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