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Autofocus method in digital holographic microscopy
SPIE Proceedings, 2009The determination of focused image plane is the key of numerical reconstruction of wavefront. In this paper, three autofocus algorithms for digital holographic microscopy, including statistical algorithm, histogram-based algorithms and Fourier spectrum algorithm, are studied based on experimental investigation.
Jingbo Liu +3 more
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Swept source digital holographic phase microscopy
Optics Letters, 2016We propose a swept source-based digital holographic phase microscopy technique. By scanning source wavelength, a series of on-axis interferograms can be obtained for accurate determination of the sample phase using spectral domain interferometry. With these sample spectra, sources of undesirable interference artifacts, often significant in holographic ...
Kiho Lee +3 more
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Digital holographic microscopy applied to neurociences
2012 11th Euro-American Workshop on Information Optics, 2012Quantitative phase imaging techniques including digital holographic microscopy (DHM) have emerged recently in life sciences and can be aimed at monitoring and quantifying, in a non-invasive way, cellular processes. Due to its interferometric nature, the DHM phase signal or optical path difference (OPD), which depends on both cell thickness and integral
Pascal Jourdain +5 more
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Compressed sensing for digital holographic microscopy
2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 2010This paper describes an original microscopy imaging framework successfully employing Compressed Sensing for digital holography. Our approach combines a sparsity minimization algorithm to reconstruct the image and digital holography to perform quadrature-resolved random measurements of an optical field in a diffraction plane.
Jean-Christophe Olivo-Marin +3 more
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Self-referenced digital holographic microscopy
2012 13th International Workshop on Cellular Nanoscale Networks and their Applications, 2012By developing a self referenced digital holographic microscope it becomes possible to record holograms and numerically reconstruct volumetric images of low coherence fluorescent objects such as (auto)fluorescent biological samples (e.g. algae). Our goal was to develop and construct a simple, compact portable device.
Szabolcs Tokes +2 more
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Multi-dimensional digital holographic microscopy
Optical Design and Testing VIII, 2018In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent ...
Manoj Kumar +3 more
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Detection of airborne nanoparticles with lateral shearing digital holographic microscopy
Optics and lasers in engineering, 2022Gülhan Ustabaş Kaya +6 more
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Multiple-wavelength digital holographic microscopy
2009In this thesis work, we extend digital holographic microscopy (DHM) capabilities and applications range by using from two to twenty different wavelength laser sources, either simultaneously or sequentially. As an overview, we present methods to increase the measurement range to more than three decades, from demonstrated sub-nanometer precision to ...
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