Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy [PDF]
This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment.
Absil+34 more
core +2 more sources
Principles and techniques of digital holographic microscopy [PDF]
Digital holography is an emerging field of new paradigm in general imaging applications. We present a review of a subset of the research and development activities in digital holography, with emphasis on microscopy techniques and applications. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are ...
Myung K. Kim
openalex +2 more sources
Monitoring Human Neutrophil Differentiation by Digital Holographic Microscopy
We report on the usefulness of digital holographic microscopy (DHM) for the assessment of human neutrophil differentiation from myeloid cells. The cell and nuclear regions have been designated by image segmentation of the optical phase function, and the ...
Maria Augusta do R. B. F. Lima, D. Cojoc
semanticscholar +3 more sources
Digital holographic microscopy for real-time observation of surface-relief grating formation on azobenzene-containing films. [PDF]
Light-induced surface structuring of azobenzene-containing films allows for creation of complex surface relief patterns with varying heights, patterns which would be difficult to create using conventional lithography tools.
Rekola H+4 more
europepmc +2 more sources
Holographic microscopy for the three-dimensional exploration of light scattering from gold nanomarkers in biological media [PDF]
The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and the phase ...
Abboud, Marie+4 more
core +2 more sources
Wavefront division digital holographic microscopy. [PDF]
Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable ...
Patel N+7 more
europepmc +6 more sources
Digital holographic microscopy for the evaluation of human sperm structure [PDF]
SummaryThe morphology of the sperm head has often been correlated with the outcome ofin vitrofertilization, and has been shown to be the sole parameter in semen of value in predicting the success of intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection.In this paper, we have studied whether digital holographic ...
G. Coppola+8 more
openaire +7 more sources
Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays. [PDF]
3D cell culture assays are becoming increasingly popular due to their higher resemblance to tissue environment. These provide an increased complexity compared to the growth on 2D surface and therefore allow studies of advanced cellular properties such as
Hellesvik M, Øye H, Aksnes H.
europepmc +2 more sources
An Inexpensive Portable Self-Reference Module for Digital Holographic Microscopy
This paper describes a novel optical system that can be integrated to the image port of an existing brightfield microscope in order to enhance the microscope with the features of digital holographic microscopy. The proposed system is modular and portable.
Xin Fan+3 more
semanticscholar +3 more sources
Polychromatic digital holographic microscopy: a quasicoherent-noise-free imaging technique to explore the connectivity of living neuronal networks. [PDF]
. Significance: Over the past decade, laser-based digital holographic microscopy (DHM), an important approach in the field of quantitative-phase imaging techniques, has become a significant label-free modality for live-cell imaging and used particularly ...
Larivière-Loiselle C+2 more
europepmc +2 more sources