Results 51 to 60 of about 5,322 (221)

Harnessing Ultrafast Optical Pulses for 3D Microfabrication by Selective Tweezing and Immobilization of Colloidal Particles in an Integrated System

open access: yesAdvanced Photonics Research, Volume 6, Issue 5, May 2025.
Microfabrication using nano‐ to micron‐sized blocks has transformative potential for next‐gen electronics, optoelectronics, and materials. Traditional methods are limited by scalability and precision. STIC, a single‐laser system for precise colloid manipulation and immobilization using femtosecond lasers, is introduced that enables efficient 3D ...
Krishangi Krishna   +4 more
wiley   +1 more source

Fast and robust diffraction based overlay metrology using dark-field digital holographic microscopy

open access: yes, 2021
Digital holographic microscopy allows access to the complex electric field, enabling computational propagation of light. This enables computational corrections for lens aberrations, which remove the requirement for antireflective coatings on the lens and
Lehmann, Peter   +10 more
core   +1 more source

An Inexpensive Portable Self-Reference Module for Digital Holographic Microscopy

open access: yesPhotonics, 2021
This paper describes a novel optical system that can be integrated to the image port of an existing brightfield microscope in order to enhance the microscope with the features of digital holographic microscopy. The proposed system is modular and portable.
Xin Fan   +3 more
doaj   +1 more source

Toward Intelligent Multimodal Holography for Real‐Time Chemical Imaging of Dynamic Ion Separation

open access: yesAdvanced Intelligent Discovery, EarlyView.
Intelligent multimodal holography integrates digital off‐axis holography, spectroscopic imaging, and AI‐driven reconstruction to visualize ion transport and chemical dynamics in real time. In this perspective paper, we outline how this approach enables label‐free, chemically specific monitoring of complex environments and discuss its potential to ...
Giovanna Ricchiuti   +3 more
wiley   +1 more source

Holography : The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

open access: yes, 2017
Digital holographic (DH) microscopy is a digital high-resolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells.
Gjörloff Wingren, Anette   +11 more
core   +1 more source

Clinically Informed Intelligent Classification of Ovarian Cancer Cells by Label‐Free Holographic Imaging Flow Cytometry

open access: yesAdvanced Intelligent Systems, Volume 7, Issue 3, March 2025.
Quantitative phase maps of single cells recorded in flow cytometry modality feed a hierarchical architecture of machine learning models for the label‐free identification of subtypes of ovarian cancer. The employment of a priori clinical information improves the classification performance, thus emulating the clinical application of liquid biopsy during ...
Daniele Pirone   +11 more
wiley   +1 more source

Digital holographic microscopy in human sperm imaging [PDF]

open access: yesJournal of Assisted Reproduction and Genetics, 2011
The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT).DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined.
Igor, Crha   +5 more
openaire   +2 more sources

Quantitative and Dynamic Phase Imaging of Biological Cells by the Use of the Digital Holographic Microscopy Based on a Beam Displacer Unit

open access: yesIEEE Photonics Journal, 2018
The digital holographic microscopy can be used for the quantitative and dynamic phase imaging of biological cells, which has a very wide application in the fields of biological and medical sciences. To make the measurement system simple, compact, and low-
Jianglei Di   +3 more
doaj   +1 more source

Profiling Co‐Occurrent Morphological Phenotypes and Their Degree of Expression Severity in Vacuolated Cells by Holo‐Tomographic Flow Cytometry and Fractal Analysis

open access: yesAdvanced Intelligent Systems, EarlyView.
HTFC gets 3D refractive index tomograms of flowing cells. Label‐free monocytes are engineered to express patterns of cytoplasmic vacuoles. From the tomogram, an efficient dimensionality reduction is operated. Interpretable features are extracted to classify the expression severity of phenotypes coexisting in each cell, visually represented by a seven ...
Marika Valentino   +9 more
wiley   +1 more source

Structured illumination lensless digital holographic microscopy (SI-LDHM)

open access: yesFrontiers in Physics
In this work, we propose a structured-illumination lensless digital holographic microscopy (SI-LDHM). SI-LDHM illuminates a sample with 24 structured illuminations (8 orientations × 3 phase shifts) and records the defocused interferogram formed by two ...
Juanjuan Zheng   +23 more
doaj   +1 more source

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