Results 121 to 130 of about 39,171,051 (331)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
More than Semantics? Navigating the “Policy * Design” Concepts’ Landscape
Central European Journal of Public PolicyScholarship from the design discipline and policy sciences has produced rich empirical and theoretical knowledge on the intersection of policy and design.
Villa-Alvarez Diana Pamela +1 more
doaj +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Digital Transitions: Nonprofit Investigative Journalism: Evaluation Report on the Center for Public Integrity [PDF]
, 2010 Summarizes outcomes of a one-year grant to CPI to transform itself into a leader in digital nonprofit journalism. Examines CPI's track record, use of new tools and methods, capacity as an effective and credible online presence, and areas for ...
Judy J. Miller +2 more
core
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Use of Digital Methods to Optimize Visualization during Surgical Gonioscopy. [PDF]
J Clin Med, 2023 Sandali O +7 more
europepmc +1 more source
THE EFFECT OF DIGITAL MARKETING MIX ON CONSUMERS PURCHASESINTENTION [PDF]
, 2019 The Digital Marketing Mix is one of the elements used by marketers in marketing their products aimed at building consumers purchase intention. Digital marketing provides a framework for comparing existing services with competitors and can also be used as
Kadarisman, A. (Asep ) +1 more
core
Ethics Issues in Digital Methods Research
, 2016 This chapter addresses issues of ethical research practice in the context of the recently emerging range of methods which use the Internet to support the creation of primary research data — variously referred to as online methods, digital methods and ...
Claire Hewson
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source