Formation of reactive oxygen species by human and bacterial pyruvate and 2- oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components [PDF]
Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols.
Adam-Vizi +123 more
core +1 more source
The tellurium oxyanion tellurite (TeO32-) is extremely harmful for most organisms. It has been suggested that a potential bacterial tellurite resistance mechanism would consist of an enzymatic, NAD(P)H-dependent, reduction to the less toxic form ...
Mauricio Arenas-Salinas +13 more
doaj +1 more source
Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels [PDF]
Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these ...
Benne R. +29 more
core +1 more source
Antigen-specific acquired immunity in human brucellosis: implications for diagnosis, prognosis, and vaccine development. [PDF]
Brucella spp., are Gram negative bacteria that cause disease by growing within monocyte/macrophage lineage cells. Clinical manifestations of brucellosis are immune mediated, not due to bacterial virulence factors.
Alessandro eSette +8 more
core +1 more source
Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31→q32 [PDF]
The gene for human dihydrolipoamide dehydrogenase (DLD) has been localized to the long arm of chromosome 7, within bands q31→q32, by gel-blot hybridization analysis with DNA from a panel of somatic cell hybrids containing various portions of human ...
Otulakowski, G +3 more
core +1 more source
Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development. [PDF]
Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits.
Blumwald, Eduardo +8 more
core +3 more sources
M1 macrophages enter a glycolytic state when endogenous nitric oxide (NO) reprograms mitochondrial metabolism by limiting aconitase 2 and pyruvate dehydrogenase (PDH) activity. Here, we provide evidence that NO targets the PDH complex by using lipoate to
Erika M. Palmieri +11 more
doaj +1 more source
Lipoic acid is a conserved cofactor necessary for the activation of several critical enzyme complexes in the aerobic metabolism of 2-oxoacids and one-carbon metabolism. Lipoate metabolism enzymes are key for lipoic acid biosynthesis and salvage.
Jin Jin +8 more
doaj +1 more source
Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms [PDF]
The presence of mitochondrial respiratory complex I in the pathogenic bloodstream stages of Trypanosoma brucei has been vigorously debated: increased expression of mitochondrially encoded functional complex I mRNAs is countered by low levels of enzymatic
Acestor +72 more
core +1 more source
Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni [PDF]
Detailed gel images of the differential spots identified by mass spectrometry and database search. Magnification of the 2D gel-areas corresponding to the 19 differentially expressed spots identified by mass spectrometry and database search.
Alba Cortés +5 more
core +5 more sources

