Results 31 to 40 of about 10,757 (125)

Formation of reactive oxygen species by human and bacterial pyruvate and 2- oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components [PDF]

open access: yes, 2015
Individual recombinant components of pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes (PDHc, OGDHc) of human and Escherichia coli (E. coli) origin were expressed and purified from E. coli with optimized protocols.
Adam-Vizi   +123 more
core   +1 more source

Flavoprotein-mediated tellurite reduction: structural basis and applications to the synthesis of tellurium-containing nanostructures

open access: yesFrontiers in Microbiology, 2016
The tellurium oxyanion tellurite (TeO32-) is extremely harmful for most organisms. It has been suggested that a potential bacterial tellurite resistance mechanism would consist of an enzymatic, NAD(P)H-dependent, reduction to the less toxic form ...
Mauricio Arenas-Salinas   +13 more
doaj   +1 more source

Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels [PDF]

open access: yes, 2001
Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these ...
Benne R.   +29 more
core   +1 more source

Antigen-specific acquired immunity in human brucellosis: implications for diagnosis, prognosis, and vaccine development. [PDF]

open access: yes, 2012
Brucella spp., are Gram negative bacteria that cause disease by growing within monocyte/macrophage lineage cells. Clinical manifestations of brucellosis are immune mediated, not due to bacterial virulence factors.
Alessandro eSette   +8 more
core   +1 more source

Localization of the human dihydrolipoamide dehydrogenase gene (DLD) to 7q31→q32 [PDF]

open access: yes, 1991
The gene for human dihydrolipoamide dehydrogenase (DLD) has been localized to the long arm of chromosome 7, within bands q31→q32, by gel-blot hybridization analysis with DNA from a panel of somatic cell hybrids containing various portions of human ...
Otulakowski, G   +3 more
core   +1 more source

Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development. [PDF]

open access: yes, 2011
Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits.
Blumwald, Eduardo   +8 more
core   +3 more sources

Pyruvate dehydrogenase operates as an intramolecular nitroxyl generator during macrophage metabolic reprogramming

open access: yesNature Communications, 2023
M1 macrophages enter a glycolytic state when endogenous nitric oxide (NO) reprograms mitochondrial metabolism by limiting aconitase 2 and pyruvate dehydrogenase (PDH) activity. Here, we provide evidence that NO targets the PDH complex by using lipoate to
Erika M. Palmieri   +11 more
doaj   +1 more source

A Novel Lipoate-Protein Ligase, Mhp-LplJ, Is Required for Lipoic Acid Metabolism in Mycoplasma hyopneumoniae

open access: yesFrontiers in Microbiology, 2021
Lipoic acid is a conserved cofactor necessary for the activation of several critical enzyme complexes in the aerobic metabolism of 2-oxoacids and one-carbon metabolism. Lipoate metabolism enzymes are key for lipoic acid biosynthesis and salvage.
Jin Jin   +8 more
doaj   +1 more source

Enigmatic presence of mitochondrial complex I in Trypanosoma brucei bloodstream forms [PDF]

open access: yes, 2012
The presence of mitochondrial respiratory complex I in the pathogenic bloodstream stages of Trypanosoma brucei has been vigorously debated: increased expression of mitochondrially encoded functional complex I mRNAs is countered by low levels of enzymatic
Acestor   +72 more
core   +1 more source

Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni [PDF]

open access: yes, 2016
Detailed gel images of the differential spots identified by mass spectrometry and database search. Magnification of the 2D gel-areas corresponding to the 19 differentially expressed spots identified by mass spectrometry and database search.
Alba Cortés   +5 more
core   +5 more sources

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