Results 61 to 70 of about 9,017 (241)

Pyruvate dehydrogenase operates as an intramolecular nitroxyl generator during macrophage metabolic reprogramming

Nature Communications, 2023
M1 macrophages enter a glycolytic state when endogenous nitric oxide (NO) reprograms mitochondrial metabolism by limiting aconitase 2 and pyruvate dehydrogenase (PDH) activity. Here, we provide evidence that NO targets the PDH complex by using lipoate to
Erika M. Palmieri, Ronald Holewinski, Christopher L. McGinity, Ciro L. Pierri, Nunziata Maio, Jonathan M. Weiss, Vincenzo Tragni, Katrina M. Miranda, Tracey A. Rouault, Thorkell Andresson, David A. Wink, Daniel W. McVicar   +11 more
doaj   +1 more source

Kinetic Model for a Threshold Filter in an Enzymatic System for Bioanalytical and Biocomputing Applications [PDF]

J. Phys. Chem. B 118, 12435-12443 (2014), 2014
A recently experimentally observed biochemical "threshold filtering" mechanism by processes catalyzed by the enzyme malate dehydrogenase is explained in terms of a model that incorporates an unusual mechanism of inhibition of this enzyme that has a reversible mechanism of action.
arxiv   +1 more source

Differences in gene expression of enzymes involved in branched-chain amino acid metabolism of abdominal subcutaneous adipose tissue between pregnant women with and without PCOS

Taiwanese Journal of Obstetrics & Gynecology, 2021
Objective: Polycystic ovary syndrome (PCOS) appears to be a common endocrine disorder of women in reproductive age. Adipose tissue (AT) is known as an active tissue in the metabolism of branched-chain amino acids (BCAA; Valine, Leucine, and Isoleucine ...
Shekoufeh Hajitarkhani, Ashraf Moini, Maryam Hafezi, Maryam Shahhoseini, AliReza Alizadeh   +4 more
doaj  

GC-MS Profile of Diodella sarmentosa (SW) Bacigalupo El Cabral ex Borhidi Ethanol Leaf Extract and its Total Dehydrogenase Inhibitory Potential [PDF]

arXiv, 2021
Phytochemical composition of ethanol leaf extract of Diodella sarmentosa was profiled with GC-MS and the inhibitory property of the extract against total microbial dehydrogenases were assessed. The major constituents of the extract were squalene (29.50%), Phytol (24.68%), phenol, 3-pentadecyl- (18.58%), 1-Butanol, 3-methyl- (9.09%) and n-Hexadecanoic ...
arxiv  

Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system [PDF]

Journal of Bacteriology, 1991
Dihydrolipoamide dehydrogenase (DHLDH), dihydrolipoamide acetyltransferase (DHLTA), and acetoin: 2,6-dichlorophenolindophenol oxidoreductase (Ao:DCPIP OR) were purified from acetoin-grown cells of Pelobacter carbinolicus. DHLDH had a native Mr of 110,000, consisted of two identical subunits of Mr 54,000, and reacted only with NAD(H) as a coenzyme.
Bernhard Schmidt, Fred Bernd Oppermann, Alexander Steinbüchel   +2 more
openaire   +3 more sources

Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress [PDF]

, 2002
The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory
Beliaev, Alexander S., Brandt, Craig C., Giometti, Carol S., Khare, Tripti, Lies, Douglas P., Lim, Hanjo, Nealson, Kenneth H., Thompson, Dorothea K., Tiedje, James M., Tollaksen, Sandra L., Yates, John, III, Zhou, Jizhong   +11 more
core   +2 more sources

Analysis of Secreted Proteins and Potential Virulence via the ICEs-Mediated Pathway of the Foodborne Pathogen Vibrio parahaemolyticus

Frontiers in Microbiology, 2021
Vibrio parahaemolyticus uses bacterial secretion systems and integrative and conjugative elements (ICEs) to induce various diseases and to adapt to harsh environments, respectively.
Yu He, Yu He, Shuai Wang, Shuai Wang, Kaiwen Wang, Jinwei Zhou, Jinwei Zhou, Zhi Han, Fengjiao Sun   +8 more
doaj   +1 more source

Collaborative learning of images and geometrics for predicting isocitrate dehydrogenase status of glioma [PDF]

arXiv, 2022
The isocitrate dehydrogenase (IDH) gene mutation status is an important biomarker for glioma patients. The gold standard of IDH mutation detection requires tumour tissue obtained via invasive approaches and is usually expensive. Recent advancement in radiogenomics provides a non-invasive approach for predicting IDH mutation based on MRI.
arxiv  

Evaluation of Shifts of Gene Transcription Levels of Unicellular Green Alga Chlamydomonas reinhardtii Due to UV-C Irradiation

Microorganisms, 2023
Green algae produce valuable lipids as carbon-recycling resources. Collecting whole cells with the intracellular lipids could be efficient without cell burst; however, direct use of the cells causes microbial contamination in environments.
Akihito Nakanishi, Nanami Ozawa, Masahiko Watanabe   +2 more
doaj   +1 more source

Folding of the Protein Domain hbSBD [PDF]

Biophysical J. 89, 3353 (2005), 2006
The folding of the alpha-helice domain hbSBD of the mammalian mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex is studied by the circular dichroism technique in absence of urea. Thermal denaturation is used to evaluate various thermodynamic parameters defining the equilibrium unfolding, which is well described by the two-state ...
arxiv   +1 more source