Results 81 to 90 of about 46,136 (329)

Engineering of 2‐ketoacid Decarboxylases for Production of Isobutanol and Other Fusel Alcohols in Saccharomyces cerevisiae

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Isobutanol is a fusel alcohol that can be produced microbially for use as a biofuel or upgraded into sustainable aviation fuel (SAF). A key enzyme in the isobutanol biosynthetic pathway is 2‐ketoacid decarboxylase (KDC), which irreversibly decarboxylates 2‐ketoisovalerate (KIV) to yield isobutyraldehyde.
Joshua J. Dietrich, Maelia Dziedzic, Jia Sun, Sri Harsha Adusumilli, Carla Gonçalves, Chris Todd Hittinger, Brian F. Pfleger   +6 more
wiley   +1 more source

Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose In vitro viability of banana pollen grain under different concentrations of boric acid and sucrose

Ciência e Agrotecnologia, 2011
Neste trabalho, objetivou-se avaliar a germinação do grão de pólen e o comprimento do tubo polínico das bananeiras diplóides M53, 8987-01 e 9197-03, Calcutá, Lidi e 86B79-12.
Ronaldo Viana dos Reis, Lucymeire de Souza Morais-Lino, Sebastião de Oliveira e Silva, Edson Perito Amorim, Carlos Alberto da Silva Ledo, Alexandre Pio Viana   +5 more
doaj  

Clonal growth characteristics and diversity patterns of different Clintonia udensis (Liliaceae) diploid and tetraploid cytotypes in the Hualongshan Mountains

Scientific Reports
Polyploidization plays an important role in plant evolution and biodiversity. However, intraspecific polyploidy compared to interspecific polyploidy received less attention. Clintonia udensis (Liliaceae) possess diploid (2n = 2x = 14) and autotetraploid (
Mian Han, Qiyang Qie, Meilan Liu, Huiqin Meng, Tiantian Wu, Yadi Yang, Lingling Niu, Genlou Sun, Yiling Wang   +8 more
doaj   +1 more source

Mechanism of auxin and GA crosstalk in diploid strawberry fruit initiation [PDF]

, 2020
Junhui Zhou, John Sittmann, Lei Guo, Xiaolong Huang, Anuhya Pulapaka, Zhongchi Liu   +5 more
openalex   +1 more source

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, EarlyView.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

Nuclear mutations affecting mitochondrial structure and function in Chlamydomonas [PDF]

, 1977
Wild type cells of the green alga Chlamydomonas reinhardtii can grow in the in the dark by taking up and respiring exogenously supplied acetate. Obligate photoautotrophic (dark dier, dk) mutants of this alga have been selected which grow at near wild ...
Boynton, J. E., Gillham, N. W., Wiseman, Andrew   +2 more
core   +1 more source

Evaluation of gene editing in CHO cells using the Cas‐CLOVER system

Biotechnology Progress, EarlyView.
Abstract Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the programmable nuclease (PN) Cas‐CLOVER to precisely target the Glutamine synthetase (GS) locus in CHO cells. A
Tiffany McLamarrah, Efecan Aral, Michael Hoffman, Jennifer Tedstone, Thomas King, Jason Vitko, Maria João Sebastião, Jose M. Escandell, Mafalda M. Dias, Iona McCall, Daniel Machado, Victor Cairns, Christine DeMaria, John J. Scarcelli   +13 more
wiley   +1 more source

Comparing physiological responses of acute and chronically stressed diploid and triploid Atlantic salmon (Salmo salar)

Aquaculture Reports
We compared the performance and physiological response of diploid and triploid Atlantic salmon (Salmo salar) following 21 days of either unpredictable chronic stress (UCS) or no disturbance (control).
Angelico Madaro, Floriana Lai, Per Gunnar Fjelldal, Tom Hansen, Virginie Gelebart, Brynjar Muren, Ivar Rønnestad, Rolf Erik Olsen, Lars Helge Stien   +8 more
doaj   +1 more source

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Cultivars Ploidy Influence on Variability of Some Red Clover Traits Important for Agriculture and Medicinal Purpose

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
Red clover also known as Trifolium pratense L., is a perennial allogame plant belonging to Trifolium genus and Fabaceae family. Besides its benefits in animal feed and soil improvement, red clover was studied as well for its medicinal properties, being ...
Alexandru D. COSTIN, Leon MUNTEAN, Andreea D. ONA, Mihai C. POPA   +3 more
doaj   +1 more source