Results 101 to 110 of about 3,265,997 (303)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

THEORETICAL AND PRACTICAL CONSIDERATIONS REGARDING THE COST CALCULATION USING DIRECT COSTING [PDF]

open access: yes, 2020
: The definition of the cost of production as applied to inventories refers to the acquisition and production cost, and its determination involves many considerations.
Bunea -Bonta, Cristina Aurora
core  

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

open access: yesFEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss   +7 more
wiley   +1 more source

Perchè ricordarci del direct costing?

open access: yes, 2007
In questo studio si affronta un problema molto importante per il calcolo economico, tanto per le decisioni relative alle produzioni quanto per il controllo dell'efficienza economica e produttiva: il problema di come correlare significativamente i costi ...
MELLA, PIERO
core  

Standard costing and scientific management [PDF]

open access: yes, 1978
Many have suggested that scientific management had a direct influence on the development of standard costing. This paper examines the relationship between these concepts in broad terms.
Hallbauer, Rosalie C.
core  

CONSIDERATIONS REGARDING THE PURPOSE OF DIRECT COSTING METHOD IN A COMPANY`S MANAGEMENT [PDF]

open access: yesAnalele Universităţii Constantin Brâncuşi din Târgu Jiu : Seria Economie, 2013
This study pertains to the scope of managerial accounting and is based on the establishment and control of costs and performances within the entities in the industry of chemical fertilizers.
Ionescu Ion
doaj  

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

A ATAS PERANAN DIRECT COSTING DALAM PERENCANAAN LABA DAN PENGAMBILAN KEPUTUSAN PADA PT EKSTRINDO LAMINASI [PDF]

open access: yes, 2002
A ATAS PERANAN DIRECT COSTING DALAM PERENCANAAN LABA DAN PENGAMBILAN KEPUTUSAN PADA PT EKSTRINDO ...
IKMEI, IKMEI
core  

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