Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis [PDF]
Emerging Infectious Diseases, 2002 A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B ...
Barun K. De +11 more
doaj +6 more sources
Direct fluorescent-antibody testing followed by culture for diagnosis of 2009 H1N1 influenza A. [PDF]
J Clin Microbiol, 2011 ABSTRACT During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%.
Bakerman P +5 more
europepmc +7 more sources
Detection of Respiratory Syncytial Virus using Direct Fluorescent Antibody Assay in Paediatric Patients with Acute Respiratory Tract Infection [PDF]
Journal of Clinical and Diagnostic Research, 2016 Introduction: Severe Respiratory Syncytial Virus (RSV) pulmonary disease manifesting as bronchiolitis and pneumonia continues to play a major role in the childhood mortality and morbidity.
Roopa Bhandary, Rekha Boloor
doaj +3 more sources
Evaluation of a Semiautomated System for Direct Fluorescent Antibody Detection of Salmonellae [PDF]
Applied Microbiology, 1975 A semi-automatic system under development by Aerojet Medical and Biological Systems for the direct fluorescent antibody detection of salmonellae was evaluated with various food, feed, and environmental samples. All samples were simultaneously examined by Automated Bioassay System (ABS), manual direct fluorescent antibody procedures and cultural ...
Berenice M. Thomason +2 more
semanticscholar +6 more sources
Direct selection of functional fluorescent-protein antibody fusions by yeast display.
PLoS ONE, 2023 Antibodies are important reagents for research, diagnostics, and therapeutics. Many examples of chimeric proteins combining the specific target recognition of antibodies with complementing functionalities such as fluorescence, toxicity or enzymatic ...
Nileena Velappan +11 more
doaj +5 more sources
Evaluation of a Commercial Direct Fluorescent-Antibody Assay for Human Metapneumovirus in Respiratory Specimens [PDF]
Journal of Clinical Microbiology, 2008 ABSTRACT The performance of a commercially manufactured direct fluorescent-antibody assay kit for human metapneumovirus was compared to that of reverse transcriptase PCR, the current “gold standard.” The kit demonstrated a sensitivity of 95.2%, a specificity of 100%, and an accuracy of 98.9%.
Donald C. Vinh +3 more
semanticscholar +5 more sources
Direct Fluorescent-Antibody Technique for the Microbiological Examination of Food and Environmental Swab Samples for Salmonellae [PDF]
Applied Microbiology, 1973 Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection of Salmonella were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%
N. F. Insalata +2 more
semanticscholar +5 more sources
Comparison of Immunohistochemistry, Electron Microscopy, and Direct Fluorescent Antibody Test for the Detection of Bovine Coronavirus [PDF]
Journal of Veterinary Diagnostic Investigation, 1998 Bovine coronavirus (BCV) is 1 of the major causes of calf diarrhea and has also been implicated in respiratory infections of young calves and winter dysentery of adult cattle. Currently, transmission electron microscopy (TEM), direct fluorescent antibody (DFA), and enzyme-linked immunosorbent assay (ELISA) techniques are considered standard methods for
Arshud Dar, Sanjay Kapil, Sagar M. Goyal
semanticscholar +6 more sources
Neisseria gonorrhoeae Identification in Direct Smears by a Fluorescent Antibody-Counterstain Method [PDF]
Applied Microbiology, 1965 Direct smears from female patients have been considered unreliable for the detection of Neisseria gonorrhoeae by fluorescent-antibody (FA) methods because of inadequate background contrast of the fluorescent-stained smears and a scarcity of organisms on the smear. Evans blue dye employed as a counterstain eliminated
L A White, Douglas S. Kellogg
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