Cross-Talk-Free Multi-Color STORM Imaging Using a Single Fluorophore [PDF]
Multi-color stochastic optical reconstruction microscopy (STORM) is routinely performed; however, the various approaches for achieving multiple colors have important caveats. Color cross-talk, limited availability of spectrally distinct fluorophores with
Borbely, Joseph Steven +4 more
core +3 more sources
DETECTION OF BOVINE RESPIRATORY SYNCYTIAL VIRUS IN CALVES OF RIO GRANDE DO SUL, BRAZIL
During 20 months of the 1987-1990 period, lung tissue samples from 351 calves were obtained at a slaughterhouse. These calves were from counties nearby Porto Alegre. The direct and indirect fluorescent antibody tests (FAT) using polyclonal and monoclonal
Ivan Paulo Demartini Gonçalves +5 more
doaj +1 more source
Direct and indirect fluorescent antibody identification of yellowtail pathogens.
Direct and indirect fluorescent antibody techniques were evaluated as methods for identifica-tion of yellowtail pathogens, Streptococcus sp., Pasteurella piscicida, Nocardia kampachi and Vibrio anguillarum (serotype J-O-3) in this study. Four antibody solutions were prepared against these pathogens for direct fluorescence tests. For indirect technique,
Riichi Kusuda, Eijiro Kawahara
openaire +2 more sources
Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system [PDF]
BACKGROUND: Eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens.
Blake, D P +6 more
core +3 more sources
Cancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules
Imaging agents and drug carriers are commonly targeted toward cancer cell through functionalization with specific recognition molecules. Quantum dots (QDs) are fluorescent semiconductor nanocrystals whose extraordinary brightness and photostability make ...
Galina Nifontova +9 more
doaj +1 more source
Immuno-gold Labelling of Chlamydia trachomatis [PDF]
Background Chlamydia trachomatis is considered as an important cause of preventable sexually transmitted diseases worldwide. It is known to be of an obligate intracellular nature and enters its target cells via an endocytic process.
Saeid Hosseinzadeh, Mehdi Fazeli
doaj +1 more source
COMPARING THE SENSITIVITY OF DETECTING VIRAL ANTIGEN IN DIFFERENT PARTS OF RABIES SUSPECTED BRAIN USING FLUORESCENT ANTIBODY TEST [PDF]
Among 78 rabies suspected brain samples examined, 61 were found positive in direct fluorescent antibody test (FAT) and maximum percentage of positivity was observed (91.8 per cent) in impression smears from the brain stem.
S. Raju +2 more
doaj
To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech. [PDF]
The ability to alter neuronal gene expression, either to affect levels of endogenous molecules or to express exogenous ones, is a powerful tool for linking brain and behavior. Scientists continue to finesse genetic manipulation in mice.
Heston, Jonathan B, White, Stephanie A
core +1 more source
Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species [PDF]
BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry.
Debes, Gudrun F. +2 more
core +1 more source
Predictive Value of Serum Antibodies and Point Mutations of AQP4, AQP1 and MOG in A Cohort of Spanish Patients with Neuromyelitis Optica Spectrum Disorders [PDF]
The detection of IgG aquaporin-4 antibodies in the serum of patients with Neuromyelitis optica (NMO) has dramatically improved the diagnosis of this disease and its distinction from multiple sclerosis.
Abril-Jaramillo, Javier +14 more
core +1 more source

