Investigation of the Frequency of Cryptosporidium spp. in Immunosuppressed Patients with Diarrhea
Objective: We aimed to investigate the frequency of Cryptosporidium spp. in immunosuppressed patients who were admitted with diarrhea by microscopic, serological, and molecular methods and to evaluate the results in comparison with the direct fluorescent
Duygu Beder, Fatma Esenkaya Taşbent
doaj +1 more source
DIAGNOSIS OF PARALYTIC FORM OF RABIES IN A GOAT BY CORNEAL IMPRESSION TECHNIQUE-A CASE REPORT [PDF]
A case of paralytic form of rabies in a goat and its antemortem diagnosis by direct fluorescent antibody test on corneal impression smears was described.
P.V. Tresamol +4 more
doaj
Direct rapid immuno-histochemical test (DRIT) is used as a gold standard method for rabies virus detection. The present study aimed to compare and evaluate DRIT with direct fluorescent antibody test (DFAT) to use equivalently as one of rabies diagnosing ...
Sintayehu Abdella +15 more
doaj +1 more source
Functional selectivity of GPCR-directed drug action through location bias. [PDF]
G-protein-coupled receptors (GPCRs) are increasingly recognized to operate from intracellular membranes as well as the plasma membrane. The β 2 -adrenergic GPCR can activate G s -linked cyclic AMP (G s -cAMP) signaling from endosomes.
Conti, Marco +6 more
core +3 more sources
Distribution of tumor antigen in cell fractions of adenovirus 12-induced tumor--its application to fluorescent antibody technique [PDF]
Adl2-induced tumors were homogenized and fractionated by the SCHNEIDER'S method. The CF test with the serum from tumor-bearing hamsters revealed the predominant presence of T-antigen in the mitochondrial and microsomal fractions.
Okamoto, Tsukasa
core +1 more source
Actin, α-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells [PDF]
During the spreading of a population of rat embryo cells, approximately 40% of the cells develop a strikingly regular network which precedes the formation of the straight actin filament bundles seen in the fully spread out cells.
Lazarides, Elias
core +1 more source
PBXL-1: A New Fluorochrome Applied to Detection of Proteins on Membranes
An easy, sensitive and direct fluorescent immunodetection method for proteins is described using the new fluorochrome PBXL™-1 imaged with the FMBIO® II Laser Scanning Imaging System.
John P. Morseman +3 more
doaj +1 more source
Campylobacteriosis in beef cattle in Alentejo – preliminary assessment [PDF]
The aim of this study was to assess the prevalence of infection by Campylobacter fetus in beef cattle reared in extensive systems in Alentejo region by direct fluorescent antibody test (DFAT), a technique widely used for determining the prevalence of ...
Pinto Coelho, M +2 more
core
The subnuclear localization of tRNA ligase in yeast [PDF]
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source

