Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering [PDF]
Industrial plant biotechnology applications include the production of sustainable fuels, complex metabolites and recombinant proteins, but process development can be impaired by a lack of reliable and scalable screening methods. Here, we describe a rapid
Arfi+28 more
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Investigation of the Frequency of Cryptosporidium spp. in Immunosuppressed Patients with Diarrhea
Objective: We aimed to investigate the frequency of Cryptosporidium spp. in immunosuppressed patients who were admitted with diarrhea by microscopic, serological, and molecular methods and to evaluate the results in comparison with the direct fluorescent
Duygu Beder, Fatma Esenkaya Taşbent
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COMPARING THE SENSITIVITY OF DETECTING VIRAL ANTIGEN IN DIFFERENT PARTS OF RABIES SUSPECTED BRAIN USING FLUORESCENT ANTIBODY TEST [PDF]
Among 78 rabies suspected brain samples examined, 61 were found positive in direct fluorescent antibody test (FAT) and maximum percentage of positivity was observed (91.8 per cent) in impression smears from the brain stem.
S. Raju+2 more
doaj
Rapid, Direct Fluorescent-Antibody Method for the Detection of Salmonellae in Food and Feeds
An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide.
C. W. Mahnke+2 more
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Cell Propagation of Cholera Toxin CTA ADP-Ribosylating Factor by Exosome Mediated Transfer [PDF]
In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population.
Boussadia, Zaira+11 more
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Applying antibodies inside cells: Principles and recent advances in neurobiology, virology and oncology [PDF]
To interfere with cell function, many scientists rely on methods that target DNA or RNA due to the ease with which they can be applied. Proteins are usually the final executors of function but are targeted only indirectly by these methods.
Marschall, Andrea+4 more
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DIAGNOSIS OF PARALYTIC FORM OF RABIES IN A GOAT BY CORNEAL IMPRESSION TECHNIQUE-A CASE REPORT [PDF]
A case of paralytic form of rabies in a goat and its antemortem diagnosis by direct fluorescent antibody test on corneal impression smears was described.
P.V. Tresamol+4 more
doaj
PBXL-1: A New Fluorochrome Applied to Detection of Proteins on Membranes
An easy, sensitive and direct fluorescent immunodetection method for proteins is described using the new fluorochrome PBXL™-1 imaged with the FMBIO® II Laser Scanning Imaging System.
John P. Morseman+3 more
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Antigenic and genetic characterization of a divergent African virus, Ikoma lyssavirus [PDF]
In 2009, a novel lyssavirus (subsequently named Ikoma lyssavirus, IKOV) was detected in the brain of an African civet (Civettictis civetta) with clinical rabies in the Serengeti National Park of Tanzania.
Alejandro Nunez+21 more
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Serologic Evidence of Lyssavirus Infections among Bats, the Philippines
Active surveillance for lyssaviruses was conducted among populations of bats in the Philippines. The presence of past or current Lyssavirus infection was determined by use of direct fluorescent antibody assays on bat brains and virus neutralization ...
Paul M. Arguin+5 more
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