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Limitations of Direct Immunofluorescence
Archives of Dermatology, 1992To the Editor.— The article by Fabre et al 1 on the presence of granular deposits of immunoglobulin at the basement membrane zone of normal sun-exposed skin is valuable in pointing out some of the limitations of direct immunofluorescence for the diagnosis of lupus erythematosus.
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Automated direct immunofluorescence analyses of skin biopsies
Journal of Cutaneous Pathology, 2015BackgroundDiagnosis of autoantibody‐ and immune complex‐induced skin diseases is primarily based on direct immunofluorescence (DIF) microscopy. DIF staining is usually performed manually and, therefore, is labor intensive. The quality of immunohistochemical results considerably depends on the experience of the person conducting the tests.
Susanne, Lemcke +11 more
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Direct Immunofluorescent Labeling of Cells
2009In the direct immunofluorescent labeling technique, fluorochrome-labeled antibodies are used as probes for particular antigens or biomolecules. Cells, usually after appropriate fixation, are incubated with the antibodies to which fluorochromes have been directly conjugated.
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Direct and indirect immunofluorescent findings in dermatomyositis
Journal of Cutaneous Pathology, 1985Three skin biopsies (proximal nailfold, extensor forearm and buttock) and serum samples were studied by direct and indirect immunofluorescence, immunodiffusion and radioimmunoassay techniques in 6 patients with dermatomyositis. A variety of seroligic and immunopathologic abnormalities was observed in the patients.
Z, Chen +5 more
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Direct Cutaneous Immunofluorescent Test in Lupus Erythematosus
Southern Medical Journal, 1973Immunoglobulins are deposited at the dermal-epidermal (D-E) junction in the skin lesions of most patients with systemic and discoid lupus erythematosus. These immunoglobulins can be detected by the direct cutaneous immuno-fluorescent test, allowing early diagnosis.
L T, Nesbitt, O B, Gum
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International Journal of Dermatology, 2009
AbstractBackground Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence pattern may be present in the ORS.Methods Twenty consecutive patients of pemphigus were
Raghavendra, Rao +3 more
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AbstractBackground Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence pattern may be present in the ORS.Methods Twenty consecutive patients of pemphigus were
Raghavendra, Rao +3 more
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Punctate pemphigus: an underreported direct immunofluorescence pattern
Journal of Cutaneous Pathology, 2013Intercellular epidermal deposition of immunoglobulin G (IgG) in a continuous net‐like or ‘chicken wire’ pattern is a well‐described and diagnostic finding in direct immunofluorescence (DIF) studies of pemphigus. In our experience, punctate or dot‐like intercellular deposition of IgG can also be seen in cases of pemphigus but has received little ...
Christine J, Ko, Jennifer M, McNiff
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Limitations of Direct Immunofluorescence-Reply
Archives of Dermatology, 1992In Reply.— We appreciate Bystryn's comments and agree that a more appropriate site selection for immunofluorescence biopsy in patients in whom systemic lupus erythematosus is being considered is a less exposed area. Our study, however, was meant to address the rate of "false" positivity on an exposed surface such as the head or neck.
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Immunofluorescence directe en dermatologie : principales indications
Revue Francophone des Laboratoires, 2019Resume Le pathologiste doit se familiariser avec les principaux aspects de l'immunofluorescence directe en dermatologie, poser au mieux les indications de celle-ci, et apprendre a eviter les pieges potentiels. L'immunofluorescence directe est une technique de microscopie permettant la detection d'immunoglobulines/complexes immuns et de fractions du ...
Sébastien Menzinger +3 more
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Direct Immunofluorescence in the Diagnosis of Legionnaires' Disease
Chest, 1981Over a 2 1/2 year period, 61 clinical specimens from 41 patients with pneumonia of uncertain etiology were evaluated for the presence of Legionella pneumophila (serogroups 1 to 4) by immunofluorescent antibody techniques. In 13 of 19 patients with Legionnaires' disease, the diagnosis was established by fluorescent antibody (FA) staining of lung ...
L D, Saravolatz +2 more
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