Results 251 to 260 of about 3,477,747 (293)

Limitations of Direct Immunofluorescence

Archives of Dermatology, 1992
To the Editor.— The article by Fabre et al 1 on the presence of granular deposits of immunoglobulin at the basement membrane zone of normal sun-exposed skin is valuable in pointing out some of the limitations of direct immunofluorescence for the diagnosis of lupus erythematosus.
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Direct immunofluorescence in the diagnosis of scleroderma syndromes

British Journal of Dermatology, 1977
Immunofluorescent study of the skin of nine patients with mesenchymal, inflammatory scleroderma (mixed connective tissue disease) revealed immunoglobulin and complement deposition at the basement membrane or within blood vessel walls. The skin specimens of ten patients with systemic scleroderma were negative for immunofluorescence.
R K, Winkelmann, F J, Carapeto, R E, Jordon   +2 more
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Direct Immunofluorescence in Sural Nerve Biopsies

European Neurology, 2008
Direct immunofluorescence (DIF) has been carried out in 66 sural nerve biopsies using antibodies against human IgG, IgA, IgM, C3, C4, albumin, fibrinogen, and ĸ- and λ-chains. In 37 out of 63 (59%) different neuropathies immunoglobulins or other plasma proteins were found within the peripheral nerves.
SCHENONE, ANGELO, DE MARTINI, ISABELLA, TABATON, MASSIMO, P. Romagnoli, A. S. Akkad, D. Bianchini, MANCARDI, GIOVANNI LUIGI   +6 more
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Demonstration of pemphigus‐specific immunofluorescence pattern by direct immunofluorescence of plucked hair

International Journal of Dermatology, 2009
AbstractBackground  Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence pattern may be present in the ORS.Methods  Twenty consecutive patients of pemphigus were
Raghavendra, Rao, Kavitha, Dasari, Shrutakirthi, Shenoi, Chandrashekaran, Balachandran   +3 more
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Direct Immunofluorescent Labeling of Cells

2009
In the direct immunofluorescent labeling technique, fluorochrome-labeled antibodies are used as probes for particular antigens or biomolecules. Cells, usually after appropriate fixation, are incubated with the antibodies to which fluorochromes have been directly conjugated.
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Direct Immunofluorescence Diagnosis of Pemphigus without Biopsy

Journal of Cutaneous Medicine and Surgery, 1998
Background: Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis.
Feliciani, C., Mohammad Pour, S., Toto, P., Coscione, G., Amerio, P., Amerio, P.   +5 more
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The evaluation of a special liquid fixative for direct immunofluorescence

Clinical and Experimental Dermatology, 1977
Summary The direct immunofluorescence of bullous disorders and lupus erythematosus was compared using a liquid fixative and a standard freezing method. Sections were left in fixative for 1, 3 or 5 days before processing. The intensity of fluorescence faded and became absent in some cases where the sections were in fixative for 3 or 5 days.
M V, Skeete, M M, Black
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Cytoid bodies in cutaneous direct immunofluorescence examination

Journal of Cutaneous Pathology, 2007
Background:  The clinical significance of scattered or grouped cytoid bodies seen on direct immunofluorescence (DIF) examination has not been systematically analyzed in a variety of disorders.Methods:  We prospectively studied skin biopsy specimens obtained from July 2002 to December 2005 for which DIF examination using immunoglobulin (Ig) A, IgG, IgM,
Yu-Hung, Wu, Yang-Chih, Lin
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Automated direct immunofluorescence analyses of skin biopsies

Journal of Cutaneous Pathology, 2015
BackgroundDiagnosis of autoantibody‐ and immune complex‐induced skin diseases is primarily based on direct immunofluorescence (DIF) microscopy. DIF staining is usually performed manually and, therefore, is labor intensive. The quality of immunohistochemical results considerably depends on the experience of the person conducting the tests.
Susanne, Lemcke, Siegmar, Sokolowski, Nicole, Rieckhoff, Michael, Buschtez, Christian, Kaffka, Antje, Winter-Keil, Christine, Schaller, Norbert, Rottmann, Christian D, Sadik, Winfried, Stöcker, Detlef, Zillikens, Enno, Schmidt   +11 more
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The contribution of direct immunofluorescence to the diagnosis of lupus erythematosus

Journal of Cutaneous Pathology, 1989
The contributions of direct immunofluorescence, light microscopy and serology to the investigation of 52 patients with possible lupus erythematosus were studied. Direct immunofluorescence was found to be most valuable in the investigation of possible systemic lupus erythematosus whilst only of supportive value in the investigation of possible chronic ...
R E, Williams, R M, Mackie, R, O'Keefe, W, Thomson   +3 more
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