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Natural selection directing molecular evolution in vertebrate viral sensors
Developmental & Comparative ImmunologyDiseases caused by pathogens contribute to molecular adaptations in host immunity. Variety of viral pathogens challenging animal immunity can drive positive selection diversifying receptors recognising the infections. However, whether distinct virus sensing systems differ across animals in their evolutionary modes remains unclear. Our review provides a
Balraj Melepat, Tao Li, Michal Vinkler
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Creation of novel costimulatory ligands through directed molecular evolution
Trends in Immunology, 2002In physiologically relevant situations, activation of naive T cells is thought to require two signals, an antigen-specific signal mediated through the T-cell receptor (signal 1) and an antigen-independent costimulatory signal mediated through activating receptors also expressed on T cells (signal 2).
Jan Woraratanadharm, Mark Rubinstein
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Directional mutation pressure, mutator mutations, and dynamics of molecular evolution
Journal of Molecular Evolution, 1993Using a general form of the directional mutation theory, this paper analyzes the effect of mutations in mutator genes on the G+C content of DNA, the frequency of substitution mutations, and evolutionary changes (cumulative mutations) under various degrees of selective constraints.
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Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs
Nature Protocols, 2010G protein-coupled receptors (GPCRs) and their downstream signaling cascades contribute to most physiological processes and a variety of human diseases. Isolating the effects of GPCR activation in an in vivo experimental setting is challenging as exogenous ligands have off-target effects and endogenous ligands constantly modulate the activity of native ...
Shuyun, Dong +2 more
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Enhancing Chitosanase Activity via Directed Evolution and Its Molecular Mechanisms
Journal of Agricultural and Food ChemistryDirected evolution is a powerful strategy to enhance enzyme performance. In this study, we optimized the rationally designed chitosanase SsCsn46 (mSsCsn46) from Streptomyces sp. N174 by fusing it with the Escherichia coli OmpA signal peptide and subjecting it to directed evolution.
Yao Chen +6 more
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Directed Molecular Evolution: Bridging the Gap between Genomics Leads and Commercial Products
OMICS: A Journal of Integrative Biology, 2002Agricultural crops, engineered to express transgenic traits, have been rapidly adopted by farmers since the initial commercialization of this technology in 1996. However, despite nearly 20 years of research in agricultural biotechnology, only two product categories have achieved commercial success: plants containing ...
Michael W, Lassner, David, McElroy
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Chemical Physics, 1978
Abstract The possibility is explored of calculating the time evolution of a given initial molecular state, in the presence of sufficiently strong nonadiabatic interactions, with a fully quantum-mechanical approach. Two methods are presented. The first one is based on the determination of the molecular eigenstates, with expansion of the nuclear ...
Cimiraglia R +2 more
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Abstract The possibility is explored of calculating the time evolution of a given initial molecular state, in the presence of sufficiently strong nonadiabatic interactions, with a fully quantum-mechanical approach. Two methods are presented. The first one is based on the determination of the molecular eigenstates, with expansion of the nuclear ...
Cimiraglia R +2 more
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ACS Synthetic Biology
Random mutagenesis is an essential technique in the directed evolution of proteins and peptides, driving advancements in protein engineering and biotechnology. This review provides a critical analysis of various error-prone polymerase chain reaction (epPCR) techniques employed for random mutagenesis, highlighting their mechanisms, advantages, and ...
Yi Torng Chai +4 more
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Random mutagenesis is an essential technique in the directed evolution of proteins and peptides, driving advancements in protein engineering and biotechnology. This review provides a critical analysis of various error-prone polymerase chain reaction (epPCR) techniques employed for random mutagenesis, highlighting their mechanisms, advantages, and ...
Yi Torng Chai +4 more
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Directed molecular evolution of nitrite oxido-reductase by DNA-shuffling.
Biomedical and environmental sciences : BES, 2007To develop directly molecular evolution of nitrite oxido-reductase using DNA-shuffling technique because nitrobacteria grow extremely slow and are unable to nitrify effectively inorganic nitrogen in wastewater treatment.The norB gene coding the ndtrite oxido-reductase in nitrobacteria was cloned and sequenced.
Jun-Wen, Li +4 more
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