Results 181 to 190 of about 549,980 (345)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

The External Relations of the European Union towards the United States: How Relevant is the European Union in Constraining or Replacing U.S. Hegemony?, Jean Monnet/Robert Schuman Paper Series, Vol. 1 No. 3, September 2002 [PDF]

open access: yes
(From the introduction). The study object of this paper is the external relations of the European Union towards the United States. The core question of this essay is not the U.S.
Rivera, Roberto Domínguez.
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

A Vote for Clarity: Establishing a Federal Test for Intervention in Election-Related Disputes [PDF]

open access: yes
Increasingly, state and federal courts are asked to resolve election-related disputes, as candidates are more likely than ever before to challenge some aspect of the administration of an election in court.
Klein, Ben
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Mediation In Family Dispute: Balancing Emotional and Legal Interest

open access: yesInternational Journal For Multidisciplinary Research
Mediation is a formal, voluntary Alternate Dispute Resolution (ADR) process where a neutral, unprejudiced mediator helps disputing parties negotiate and reach a mutually respectable agreement, avoiding a court assessed decision. The parties themselves retain control over the outcome, with the mediator easing communication relating issues and exploring ...
openaire   +1 more source

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