SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Hierarchical Fuzzy Adaptive Observer-Based Fault-Tolerant Consensus Tracking for High-Order Nonlinear Multi-Agent Systems Under Actuator and Sensor Faults. [PDF]
Sensors (Basel)Zhao L, Chen S.
europepmc +1 more source
Agent-based distributed time series forecasting system
, 2015 Michał Zabłocki
openalex +1 more source
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
FEBS Open Bio, EarlyView.Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
wiley +1 more source
From the logic of coordination to goal-directed reasoning: the agentic turn in artificial intelligence. [PDF]
Front Artif IntellHaidemariam T.
europepmc +1 more source
Impact of Distributed Wind on Bulk Power System Operations in ISO-NE (Presentation)
, 2017 Carlo Brancucci +3 more
openalex +1 more source
Transponder-Aided Joint Calibration and Synchronization Compensation for Distributed Radar Systems
, 2015 Wen-Qin Wang
openalex +2 more sources
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
FEBS Open Bio, EarlyView.Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Reclassifying hospital energy demand toward industry-like requirements for hygienic and resilient indoor environments. [PDF]
Sci RepLakjiri S, Ouassaid M.
europepmc +1 more source