SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Federated generalized additive models for location, scale and shape. [PDF]
Swenne A +3 more
europepmc +1 more source
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
wiley +1 more source
Visual outcomes of scleral lenses in different stages and cone locations of keratoconus: A retrospective observational study. [PDF]
Kaya S, Sağlik A.
europepmc +1 more source
A Low-Frequency Sound Absorption Device Fitting for Power Transformation and Distribution Room
Xiaowen Wu +3 more
openalex +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Water Quality Criteria and Ecological Risk Assessment of Fluoride for the Protection of Water Organisms in Surface Water. [PDF]
Zhang J, Pu Y, Ye J, Hu X, Feng C.
europepmc +1 more source
Determining Best Fitting Distributions for Minimum Flows of Streams in Gediz Basin
Naci Büyükkaracığan
openalex +1 more source
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Research on the Coupled Bionic Design and Validation of Flying Car Folding Wings Based on Eurasian Eagle-Owl Wing Shape. [PDF]
Li Z, Cao Y, Zhao D.
europepmc +1 more source

