Results 211 to 220 of about 2,005,128 (269)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Integrating Distributed Algorithms into Distributed Systems [PDF]

open access: possibleFundamenta Informaticae, 1999
A distributed algorithm is often used as a part of a larger distributed system. Usually, the properties of an algorithm are proven for the algorithm in isolation. Then, it is not obvious how the algorithm behaves when integrated into a larger system. In this paper, we present a simple technique which allows to derive properties of an algorithm which is
Ekkart Kindler, Sibylle Peuker
openaire   +2 more sources
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Dissipative distributed systems

Proceedings of the 39th IEEE Conference on Decision and Control (Cat. No.00CH37187), 2002
Deals with systems described by constant coefficient linear partial differential equations. We define dissipativity with respect to a quadratic differential form, i.e., a quadratic functional in the system variables and their partial derivatives. The main result states the equivalence of dissipativity and the existence of a storage function or of a ...
Harish K. Pillai, Jan C. Willems
openaire   +1 more source

Distributed Systems

Computer, 1985
Growth of distributed systems has attained unstoppable momentum. If we better understood how to think about, analyze, and design distributed systems, we could direct their implementation with more confidence.
openaire   +1 more source

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