Results 11 to 20 of about 179,181 (346)

A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab

Scientific Reports, 2021
Generally, intermolecular disulfide bond contribute to the conformational protein stability. To identify sites where intermolecular disulfide bond can be introduced into the Fab’s constant domain of the therapeutic IgG, Fab mutants were predicted using ...
Hitomi Nakamura, Moeka Yoshikawa, Naoko Oda-Ueda, Tadashi Ueda, Takatoshi Ohkuri   +4 more
doaj   +1 more source

Polarity of disulfide bonds [PDF]

Protein Science, 1993
1Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 ...
A J, Saunders, G B, Young, G J, Pielak
openaire   +2 more sources

Effects of Amylopectins from Five Different Sources on Disulfide Bond Formation in Alkali-Soluble Glutenin

Foods, 2023
Wheat, maize, cassava, mung bean and sweet potato starches have often been added to dough systems to improve their hardness. However, inconsistent effects of these starches on the dough quality have been reported, especially in refrigerated dough.
Yu Zhou, Jinjin Zhao, Junjie Guo, Xijun Lian, Huaiwen Wang   +4 more
doaj   +1 more source

Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Microbial Cell Factories, 2022
Background Escherichia coli (E. coli) is a promising host for production of recombinant proteins (including antibodies and antibody fragments) that don’t require complex post-translational modifications such as glycosylation.
Tomasz K. Baginski, Karthik Veeravalli, Rebekah McKenna, Christopher Williams, Katherine Wong, Christina Tsai, Daniel Hewitt, Karthik Mani, Michael W. Laird   +8 more
doaj   +1 more source

Investigating the crucial roles of aliphatic tails in disulfide bond-linked docetaxel prodrug nanoassemblies

Asian Journal of Pharmaceutical Sciences, 2021
Disulfide bond-bridging strategy has been extensively utilized to construct tumor specificity-responsive aliphatic prodrug nanoparticles (PNPs) for precise cancer therapy.
Yuequan Wang, Cong Luo, Shuang Zhou, Xinhui Wang, Xuanbo Zhang, Shumeng Li, Shenwu Zhang, Shuo Wang, Bingjun Sun, Zhonggui He, Jin Sun   +10 more
doaj   +1 more source

Disulfide Bonds and Protein Folding [PDF]

Biochemistry, 2000
The applications of disulfide-bond chemistry to studies of protein folding, structure, and stability are reviewed and illustrated with bovine pancreatic ribonuclease A (RNase A). After surveying the general properties and advantages of disulfide-bond studies, we illustrate the mechanism of reductive unfolding with RNase A, and discuss its application ...
W J, Wedemeyer, E, Welker, M, Narayan, H A, Scheraga   +3 more
openaire   +3 more sources

Mechanisms of Disulfide Bond Formation in Nascent Polypeptides Entering the Secretory Pathway

Cells, 2020
Disulfide bonds are an abundant feature of proteins across all domains of life that are important for structure, stability, and function. In eukaryotic cells, a major site of disulfide bond formation is the endoplasmic reticulum (ER).
Philip J. Robinson, Neil J. Bulleid
doaj   +1 more source

An ultralong CDRH2 in HCV neutralizing antibody demonstrates structural plasticity of antibodies against E2 glycoprotein [PDF]

, 2020
A vaccine protective against diverse HCV variants is needed to control the HCV epidemic. Structures of E2 complexes with front layer-specific broadly neutralizing antibodies (bNAbs) isolated from HCV-infected individuals, revealed a disulfide bond ...
Adams, Bailey, Battye, Chen, Chen, de Wildt, Dowd, Emsley, Evans, Flyak, Freedman, Hsu, Kabat, Kanyavuz, Keck, Kepler, Kong, Krause, Krissinel, Law, Logvinoff, Munshaw, Osburn, Reason, Sui, Swindells, Thomson, Tzarum, Wilson, Ying, Ying, Yusim, Zibbell   +32 more
core   +2 more sources

CD44 Binding to Hyaluronic Acid Is Redox Regulated by a Labile Disulfide Bond in the Hyaluronic Acid Binding Site. [PDF]

PLoS ONE, 2015
CD44 is the primary leukocyte cell surface receptor for hyaluronic acid (HA), a component of the extracellular matrix. Enzymatic post translational cleavage of labile disulfide bonds is a mechanism by which proteins are structurally regulated by ...
Helena Kellett-Clarke, Monika Stegmann, A Neil Barclay, Clive Metcalfe   +3 more
doaj   +1 more source

The role of a disulfide bridge in the stability and folding kinetics of Arabidopsis thaliana cytochrome c6A [PDF]

, 2011
Cytochrome c 6A is a eukaryotic member of the Class I cytochrome c family possessing a high structural homology with photosynthetic cytochrome c 6 from cyanobacteria, but structurally and functionally distinct through the presence of a disulfide bond and
Arslan, Babul, Barker, Bartalesi, Beasty, Bertini, Betz, Bialek, Borgia, Brems, Carter, Chen, Chida, Christopher J. Howe, Cohen, Colon, Colon, Derek S. Bendall, Ellman, Elove, Feige, Gianni, Guidry, Gupta, Hogg, Howe, Jody M. Mason, Jonathan A.R. Worrall, Lange, Lange, Marcaida, Mason, Mason, Merchant, Mines, Molina-Heredia, Moore, Myers, Ogawa, Pace, Pace, Parrini, Pascher, Ptitsyn, Roder, Russell, Russell, Santoro, Satoh, Sauder, Schlarb-Ridley, Schmidt, Sosnick, Tezcan, Travaglini-Allocatelli, Travaglini-Allocatelli, Travaglini-Allocatelli, Ullah, Ullah, Wastl, Wastl, Wastl, Williams, Worrall, Worrall   +64 more
core   +1 more source