Results 111 to 120 of about 2,501,958 (326)

Phosphorylation of chloroplast ribulose bisphosphate carboxylase/oxygenase small subunit by an envelope-bound protein kinase in situ [PDF]

open access: yes, 1983
A new protein kinase of the cAMP independent type was found to be bound to the outer envelope membrane of spinach chloroplasts. While stimulated by Mg2+ and inhibited by ADP, the enzyme showed no response to conventional protein substrates and was ...
Buchanan, Bob B., Soll, Jürgen
core  

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies

open access: yesAdvanced Healthcare Materials, EarlyView.
Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer   +10 more
wiley   +1 more source

The Impact of Russia-Ukraine geopolitical conflict on the air quality and toxicological properties of ambient PM2.5 in Milan, Italy

open access: yesScientific Reports
The geopolitical conflict between Russia and Ukraine has disrupted Europe’s natural gas supplies, driving up gas prices and leading to a shift towards biomass for residential heating during colder months.
Yashar Aghaei   +11 more
doaj   +1 more source

Design and Synthesis of Peptide‐Polyester Conjugates for Cell‐Mediated Scaffold Degradation

open access: yesAdvanced Healthcare Materials, EarlyView.
This work describes polycaprolactone (PCL)‐based biomaterials engineered to degrade in response to cell‐secreted proteases. A fast‐degrading peptide (Fast) sequence is integrated into a PCL conjugate backbone to produce a biomaterial that is selectively degraded by multiple cell types compared to its scrambled control (ScrFast).
Korina Vida G. Sinad   +7 more
wiley   +1 more source

Seasonal Variations and Chemical Predictors of Oxidative Potential (OP) of Particulate Matter (PM), for Seven Urban French Sites

open access: yesAtmosphere, 2019
Epidemiological studies suggest that the main part of chronic effects from air pollution is likely to be linked with particulate matter (PM). Oxidative potential (OP) of PM is gaining strong interest as a promising health exposure metric.
Aude Calas   +12 more
doaj   +1 more source

An ancestral secretory apparatus in the protozoan parasite Giardia intestinalis [PDF]

open access: yes, 2003
The protozoan parasite Giardia intestinalis belongs to one of the earliest diverged eukaryotic lineages. This is also reflected in a simple intracellular organization, as Giardia lacks common subcellular compartments such as mitochondria, peroxisomes ...
Hehl, Adrian B.   +7 more
core   +1 more source

On dithiothreitol (DTT) as a measure of oxidative potential for ambient particles: evidence for the importance of soluble transition metals.

open access: yesAtmospheric Chemistry and Physics, 2012
The rate of consumption of dithiothreitol (DTT) is increasingly used to measure the oxidative potential of particulate matter (PM), which has been linked to the adverse health effects of PM. While several quinones are known to be very reactive in the DTT
Jessica G. Charrier, C. Anastasio
semanticscholar   +1 more source

Midbrain PAG Astrocytes Modulate Mouse Defensive and Panic‐Like Behaviors

open access: yesAdvanced Science, EarlyView.
Astrocytes in the midbrain periaqueductal gray (PAG) dynamically encode threat intensity and shape defensive action selection in mice. Real‐time Ca2+ imaging reveals robust astrocytic activation during predator odor and CO2 exposure. Aberrant astrocytic Ca2+ overactivation disrupts goal‐directed escape, biases behavior toward freezing, and induces ...
Ellane Barcelon   +10 more
wiley   +1 more source

Development of an immunoblot assay with infrared fluorescence to quantify paraoxonase 1 in serum and plasma

open access: yesJournal of Lipid Research, 2008
Paraoxonase 1 (PON1) requires calcium for activity and is inactivated in the presence of EDTA. Because of this, studies to date have used serum or heparinized plasma for both activity and mass assays of PON1.
Philip W. Connelly   +4 more
doaj   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

open access: yes, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Cummins, Phil   +2 more
core  

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